Topographically induced self-deformation of the nuclei of cells: dependence on cell type and proposed mechanisms

被引：40

作者：

Davidson, Patricia M. ^{[1
]}

Fromigue, Olivia ^{[2
,3
]}

Marie, Pierre J. ^{[2
,3
]}

Hasirci, Vasif ^{[4
]}

Reiter, Guenter ^{[5
]}

Anselme, Karine ^{[1
]}

机构：

[1] Univ Haute Alsace, CNRS, Inst Sci Mat Mulhouse, LRC 7228, F-68057 Mulhouse, France

[2] Hop Lariboisiere, INSERM, U606, F-75475 Paris 10, France

[3] Univ Paris Diderot, Paris, France

[4] Dept Biol Sci, Biotechnol Res Unit, METU, BIOMAT, TR-06531 Ankara, Turkey

[5] Univ Freiburg, Inst Phys, D-79104 Freiburg, Germany

来源：

JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE | 2010年 / 21卷 / 03期

关键词：

MARROW STROMAL CELLS; MECHANOTRANSDUCTION; TRANSFORMATION; DEFORMABILITY; FIBROBLASTS; EXPRESSION; PROTEINS; MATRIX; ACTIN; FORCE;

D O I：

10.1007/s10856-009-3950-7

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Osteosarcoma-derived cell lines (SaOs-2, MG63) have recently been shown to deform their nucleus considerably in response to surface topography. Such a deformation had not been described previously. Here we present results on additional cell lines, including cancerous (OHS4, U2OS), immortalized (F/STRO-1(+)A and FHSO6) and healthy cells (HOP). The cancerous cells were found to deform extensively, the immortalized cells showed small deformations, whereas the healthy cells showed deformation only at short incubation times. These results suggest a strong link between the malignant transformation of cells and the state of the cytoskeletal network. We propose mechanisms to explain the deformation in which the cytoskeleton either pushes down on the nucleus during spreading or pulls it down upon adhesion to the pillars.

引用

页码：939 / 946

页数：8

共 26 条

[1] Cellular transformation by SV40 large T antigen: interaction with host proteins [J].

Ali, SH ;

DeCaprio, JA .

SEMINARS IN CANCER BIOLOGY, 2001, 11 (01) :15-22

[2]

Anselme K, 2004, BIO-MED MATER ENG, V14, P545

[3] In vitro control of human bone marrow stromal cells for bone tissue engineering [J].

Anselme, K ;

Broux, O ;

Noel, B ;

Bouxin, B ;

Bascoulergue, G ;

Dudermel, AF ;

Bianchi, F ;

Jeanfils, J ;

Hardouin, P .

TISSUE ENGINEERING, 2002, 8 (06) :941-953

[4] THE CYTOSKELETON IN CANCER-CELLS [J].

BENZEEV, A .

BIOCHIMICA ET BIOPHYSICA ACTA, 1984, 780 (03) :197-212

[5] Nuclear matrix proteins and osteoblast gene expression [J].

Bidwell, JP ;

Alvarez, M ;

Feister, H ;

Onyia, J ;

Hock, J .

JOURNAL OF BONE AND MINERAL RESEARCH, 1998, 13 (02) :155-167

[6] Effect of Adhesion Geometry and Rigidity on Cellular Force Distributions [J].

Bischofs, Ilka B. ;

Schmidt, Sebastian S. ;

Schwarz, Ulrich S. .

PHYSICAL REVIEW LETTERS, 2009, 103 (04)

[7] Human smooth muscle α-actin gene is a transcriptional target of the p53 tumor suppressor protein [J].

Comer, KA ;

Dennis, PA ;

Armstrong, L ;

Catino, JJ ;

Kastan, MB ;

Kumar, CC .

ONCOGENE, 1998, 16 (10) :1299-1308

[8] Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography [J].

Dalby, MJ ;

Riehle, MO ;

Yarwood, SJ ;

Wilkinson, CDW ;

Curtis, ASG .

EXPERIMENTAL CELL RESEARCH, 2003, 284 (02) :274-282

[9] Microstructured Surfaces Cause Severe but Non-Detrimental Deformation of the Cell Nucleus [J].

Davidson, Patricia M. ;

Ozcelik, Hayriye ;

Hasirci, Vasif ;

Reiter, Guenter ;

Anselme, Karine .

ADVANCED MATERIALS, 2009, 21 (35) :3586-+

[10] Researching into the cellular shape, volume and elasticity of mesenchymal stem cells, osteoblasts and osteosarcoma cells by atomic force microscopy [J].

Docheva, Denitsa ;

Padula, Daniela ;

Popov, Cvetan ;

Mutschler, Wolf ;

Clausen-Schaumann, Hauke ;

Schieker, Matthias .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2008, 12 (02) :537-552

← 1 2 3 →