IGF-I and IGF-II mRNA expression in slow and fast growing families of USDA 103 channel catfish (Ictalurus punctatus)

被引：75

作者：

Peterson, BC ^{[1
]}

Waldbieser, GC ^{[1
]}

Bilodeau, L ^{[1
]}

机构：

[1] USDA ARS, Catfish Genet Res Unit, Thad Cochran Natl Warmwater Aquaculture Ctr, Stoneville, MS 38776 USA

来源：

COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY | 2004年 / 139卷 / 03期

关键词：

channel catfish; gene expression; real time per; muscle; liver; IGF-I; IGF-II;

D O I：

10.1016/j.cbpb.2004.09.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The objective of this study was to examine insulin-like growth factor (IGF)-I and IGF-II mRNA levels in fast and slow growing families of catfish. Relative levels of IGF-I and IGF-II mRNA were determined by real-time PCR. Family A exhibited a specific growth rate (SGR) of 3.6 and was designated as fast growing, while family H exhibited a SGR of 3.1 and was designated as slow growing (P=0.017). Levels of IGF-II mRNA were 3.3-fold greater (P=0.006) in muscle for the fast growing family compared to the slow growing family. Levels of IGF-II mRNA were 1.8-fold greater (P=0.049) in liver for the fast growing family compared to the slow growing family. Levels of IGF-II mRNA from both fast and slow families were 12.2-fold greater (P<0.001) in muscle and 5.8-fold greater (P=0.021) in liver, respectively, compared to levels of IGF-I mRNA. Muscle and liver levels of IGF-I mRNA were similar between families. Elevated levels of IGF-II mRNA in muscle and liver compared to IGF-I mRNA, as well as differences in levels of IGF-II mRNA between fast and slow growing families of fish suggests a role of IGF-II in growth of channel catfish. (C) 2004 Elsevier Inc. All rights reserved.

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页码：317 / 323

页数：7

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