Expression of adhesion molecules and cytokines in vitro by endothelial cells seeded on various polymer surfaces coated with titaniumcarboxonitride

被引:46
作者
Lehle, K
Buttstaedt, J
Birnbaum, DE
机构
[1] Univ Regensburg, Clin Cardiothorac Surg, D-93042 Regensburg, Germany
[2] GfE Metalle & Mat GmbH, D-90431 Nurnberg, Germany
来源
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | 2003年 / 65A卷 / 03期
关键词
endothelial cells; titanium; biocompatibility; adhesion molecules; cytokines;
D O I
10.1002/jbm.a.10492
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Although endothelial cell (EC) seeding improves the patency of vascular prostheses, the detachment of adherent ECs after the restoration of circulation remains one of the major obstacles. Polymer surfaces for endothelialization can be optimized. In this study, polyethylene terephthalate (PET), polypropylene (PP), polytetrafluoroethylene (PTFE), polyurethane (PUR), and silicone were coated with a titaniumcarboxonitride (Ti(C,N,O)) layer by a plasma-assisted chemical vapor deposition process to verify the effect of titanium onto human saphenous vein ECs. Almost confluent EC monolayers were evaluated for 1) proliferation activity and 2) expression of adhesion molecules using cellular enzyme-linked immunosorbent assay and release of cytokines. The results showed that all titanium-coated polymers and uncoated PET have no toxic effect on human saphenous vein ECs excepting uncoated PTFE, PP, and silicone. Moreover, growing ECs showed an insignificant decrease in cytokine production and an unessential change in basal expression of adhesion molecules. Tumor necrosis factor-a-induced response depends on polymer surface: for example, intercellular adhesion molecule-1 expression decreased. E-selectin expression was unchanged for culturing ECs on coated PET, PP, and PTFE and reduced for polyurethane and silicone. Vascular cell adhesion molecule-1 expression was unchanged for coated PUR and silicone and reduced for PET, PP, and PTFE. In summary, titanium-coating layers promote adhesion of human ECs on polymer vascular grafts with no proinflammatory reaction of ECs. (C) 2003 Wiley Periodicals, Inc. J Biomed Mater Res 65A: 393-401, 2003.
引用
收藏
页码:393 / 401
页数:9
相关论文
共 38 条
[1]   Coating of polymers with titanium-based layers by a novel plasma-assisted chemical vapor deposition process [J].
Breme, F ;
Buttstaedt, J ;
Emig, G .
THIN SOLID FILMS, 2000, 377 :755-759
[2]  
Breme F., 1999, MAT MED ENG, V8, P155
[3]   Cytokine production and adhesive protein expression by endothelial cells after contact with polyethylene terephthalate [J].
Cenni, E ;
Granchi, D ;
Ciapetti, G ;
Verri, E ;
Cavedagna, D ;
Gamberini, S ;
DiLeo, A ;
Pizzoferrato, A .
BIOMATERIALS, 1996, 17 (21) :2071-2076
[4]   ADHESIVE PROTEIN EXPRESSION ON ENDOTHELIAL-CELLS AFTER CONTACT IN-VITRO WITH POLYETHYLENE TEREPHTHALATE COATED WITH PYROLYTIC CARBON [J].
CENNI, E ;
GRANCHI, D ;
ARCIOLA, CR ;
CIAPETTI, G ;
SAVARINO, L ;
STEA, S ;
CAVEDAGNA, D ;
DILEO, A ;
PIZZOFERRATO, A .
BIOMATERIALS, 1995, 16 (16) :1223-1227
[5]   Expression of adhesion molecules on endothelial cells after contact with knitted Dacron [J].
Cenni, E ;
Granchi, D ;
Ciapetti, G ;
Verri, E ;
Cavedagna, D ;
Gamberini, S ;
Cervellati, M ;
DiLeo, A ;
Pizzoferrato, A .
BIOMATERIALS, 1997, 18 (06) :489-494
[6]   PRODUCTION OF PROSTACYCLIN AND FIBRINOLYSIS MODULATORS BY ENDOTHELIAL-CELLS CULTURED IN THE PRESENCE OF POLYETHYLENE TEREPHTHALATE [J].
CENNI, E ;
CIAPETTI, G ;
CAVEDAGNA, D ;
DILEO, A ;
PIZZOFERRATO, A .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH, 1993, 27 (09) :1161-1164
[7]  
Consigny PM, 2000, J LONG-TERM EFF MED, V10, P79
[8]   Adhesive protein expression on human endothelial cells after in vitro contact with woven Dacron [J].
Granchi, D ;
Cenni, E ;
Verri, E ;
Ciapetti, G ;
Gori, A ;
Gamberini, S ;
Di Leo, A ;
Pizzoferrato, A .
BIOMATERIALS, 1998, 19 (1-3) :93-98
[9]  
Guidollet J, 1999, J BIOMED MATER RES, V44, P156, DOI 10.1002/(SICI)1097-4636(199902)44:2<156::AID-JBM5>3.0.CO
[10]  
2-H