Association of Oxidative Stress with Postmenopausal Osteoporosis and the Effects of Hydrogen Peroxide on Osteoclast Formation in Human Bone Marrow Cell Cultures

被引:285
作者
Baek, Ki Hyun [3 ]
Oh, Ki Won [2 ]
Lee, Won Young [2 ]
Lee, Seong Su [3 ]
Kim, Mee Kyoung [3 ]
Kwon, Hyuk Sang [3 ]
Rhee, Eun Jung [2 ]
Han, Je Ho [3 ]
Song, Ki Ho [3 ]
Cha, Bong Yun [3 ]
Lee, Kwang Woo [3 ]
Kang, Moo Il [1 ,3 ]
机构
[1] Catholic Univ Korea, Div Endocrinol & Metab, Dept Internal Med, Seoul St Marys Hosp, Seoul 137701, South Korea
[2] Sungkyunkwan Univ, Sch Med, Dept Internal Med, Seoul, South Korea
[3] Catholic Univ Korea, Dept Internal Med, Coll Med, Seoul 137701, South Korea
关键词
Oxidative stress; Osteoporosis; 8-Hydroxy-2 '-deoxyguanosine; Bone turnover marker; Bone mineral density; Osteoclast; KAPPA-B LIGAND; DNA-DAMAGE; OSTEOBLASTIC DIFFERENTIATION; RECEPTOR ACTIVATOR; MINERAL DENSITY; OXYGEN RADICALS; METABOLISM; RESORPTION; 8-HYDROXYDEOXYGUANOSINE; BIOMARKER;
D O I
10.1007/s00223-010-9393-9
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
It has been suggested that oxidative stress is associated with the pathogenesis of osteoporosis. The objective of this study was to explore the association between a marker of oxidative stress and either bone turnover markers or bone mineral density (BMD) in postmenopausal women. In addition, the effects of oxidative stress on the formation of osteoclasts in human bone marrow cell culture were examined. We performed a cross-sectional analysis in healthy postmenopausal women aged 60-78 years (n = 135, 68.2 +/- A 4.9). Oxidative stress was evaluated in the serum by measuring 8-hydroxy-2'-deoxyguanosine (8-OH-dG) levels. The biochemical markers of bone turnover and areal BMD were measured in all participants. Multivariate linear regression analysis revealed a negative association between 8-OH-dG levels and BMD of the lumbar spine, total hip, femoral neck, and trochanter and positive association with type I collagen C-telopeptide (ICTP) levels. The odds ratio of 8-OH-dG for osteoporosis was 1.54 (1.14-2.31, P = 0.003). In cultures of primary human marrow cells, H(2)O(2) caused concentration-dependent activation of TRAP-positive multinucleated giant cells. H(2)O(2) also increased the area of pits per osteoclast activity assay substrate. RT-PCR showed that H(2)O(2) stimulated the expression of M-CSF and RANKL and increased the RANKL/OPG ratio. The data support the view that oxidative stress is associated with increased bone resorption and low bone mass in otherwise healthy women. In addition, RANKL and M-CSF stimulation induced by oxidative stress may participate in osteoclastogenesis in human bone.
引用
收藏
页码:226 / 235
页数:10
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