Epicatechin conjugated with fatty acid is a potent inhibitor of DNA polymerase and angiogenesis

被引：32

作者：

Matsubara, Kiminori ^{[1
]}

Saito, Akiko

Tanaka, Akira

Nakajima, Noriyuki

Akagi, Reiko

Mori, Masaharu

Mizushina, Yoshiyuki

机构：

[1] Okayama Prefectural Univ, Fac Hlth & Welfare Sci, Dept Nutr Sci, Okayama 7191197, Japan

[2] Kobe Gakuin Univ, Cooperat Res Ctr Life Sci, Kobe, Hyogo 6512180, Japan

[3] Toyama Prefecture, Biotechnol Ctr, Toyama 9390398, Japan

[4] Toyama Prefectural Univ, Coll Technol, Dept Bioresources Sci, Toyama 9390398, Japan

[5] Toyama Prefectural Univ, Biotechnol Res Ctr, Toyama 9390398, Japan

[6] Okayama Prefectural Univ, Dept Nursing, Fac Hlth & Welfare Sci, Okayama 7191197, Japan

[7] Kobe Gakuin Univ, Dept Nutrit Sci, Kobe, Hyogo 6512180, Japan

来源：

LIFE SCIENCES | 2007年 / 80卷 / 17期

关键词：

angiogenesis; aortic ring; epicatechin; endothelial cells; fatty acid;

D O I：

10.1016/j.lfs.2007.01.049

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Anti-cancer and anti-angiogenesis effects of green tea catechins have been demonstrated. It has been found that chemical modification of tea catechins improves their biological activities. We examined the chemical modification of epicatechin enhanced anti-cancer and anti-angiogenic effects. Epicatechin conjugated with fatty acid (acyl-catechin) strongly inhibited DNA polymerase activity, HL-60 cancer cell growth and angiogenesis. Epicatechin conjugated with palmitic acid ((2.R,3R)-3',4',5,7-tetrahydroxyflavan-3-yl hexadecanoate, epicatechin-C16) was the strongest inhibitor in DNA polymerase alpha, beta, lambda and angiogenesis assays. Epicatechin-C16 also suppressed human endothelial cell (HUVEC) tube formation on reconstituted basement membrane, suggesting that it affected not only DNA polymerase activity but also the signal transduction pathways needed for the tube formation in HUVECs. These results suggest that acylation of epicatechin is an effective chemical modification to improve the anti-cancer activity of epicatechin. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：1578 / 1585

页数：8

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