A concise guide to cDNA microarray analysis

被引：634

作者：

Hegde, P ^{[1
]}

Qi, R ^{[1
]}

Abernathy, K ^{[1
]}

Gay, C ^{[1
]}

Dharap, S ^{[1
]}

Gaspard, R ^{[1
]}

Hughes, JE ^{[1
]}

Snesrud, E ^{[1
]}

Lee, N ^{[1
]}

Quackenbush, J ^{[1
]}

机构：

[1] Inst Genom Res, Rockville, MD 20850 USA

来源：

BIOTECHNIQUES | 2000年 / 29卷 / 03期

关键词：

D O I：

10.2144/00293bi01

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Microarray expression analysis has become one of the most widely used functional genomics tools. Efficient application of this technique requires the development of robust and reproducible protocols. We have optimized all aspects of the process, including PCR amplification of target cDNA clones, microarray printing, probe labeling and hybridization, and have developed strategies for data normalization and analysis.

引用

页码：548 / +

页数：10

共 12 条

[1] ESTABLISHING A HUMAN TRANSCRIPT MAP
BOGUSKI, MS
SCHULER, GD
[J]. NATURE GENETICS, 1995, 10 (04) : 369 - 371
[2] Chen Y, 1997, J Biomed Opt, V2, P364, DOI 10.1117/12.281504
[3] Cluster analysis and display of genome-wide expression patterns
Eisen, MB
Spellman, PT
Brown, PO
Botstein, D
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (25) : 14863 - 14868
[4] Eisen MB, 1999, METHOD ENZYMOL, V303, P179
[5] DIFFERENTIAL DISPLAY OF EUKARYOTIC MESSENGER-RNA BY MEANS OF THE POLYMERASE CHAIN-REACTION
LIANG, P
PARDEE, AB
[J]. SCIENCE, 1992, 257 (5072) : 967 - 971
[6] Expression monitoring by hybridization to high-density oligonucleotide arrays
Lockhart, DJ
Dong, HL
Byrne, MC
Follettie, MT
Gallo, MV
Chee, MS
Mittmann, M
Wang, CW
Kobayashi, M
Horton, H
Brown, EL
[J]. NATURE BIOTECHNOLOGY, 1996, 14 (13) : 1675 - 1680
[7] The TIGR Gene Indices: reconstruction and representation of expressed gene sequences
Quackenbush, J
Liang, F
Holt, I
Pertea, G
Upton, J
[J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (01) : 141 - 145
[8] QUANTITATIVE MONITORING OF GENE-EXPRESSION PATTERNS WITH A COMPLEMENTARY-DNA MICROARRAY
SCHENA, M
SHALON, D
DAVIS, RW
BROWN, PO
[J]. SCIENCE, 1995, 270 (5235) : 467 - 470
[9] Pieces of the puzzle: expressed sequence tags and the catalog of human genes
Schuler, GD
[J]. JOURNAL OF MOLECULAR MEDICINE-JMM, 1997, 75 (10): : 694 - 698
[10] A DNA microarray system for analyzing complex DNA samples using two-color fluorescent probe hybridization
Shalon, D
Smith, SJ
Brown, PO
[J]. GENOME RESEARCH, 1996, 6 (07): : 639 - 645

← 1 2 →