Differences in the regulation of adipose tissue and liver lipogenesis by carbohydrates in humans

被引:92
作者
Diraison, F
Yankah, V
Letexier, D
Dusserre, E
Jones, P
Beylot, M [1 ]
机构
[1] Fac RTH Laennec, INSERM U499, F-69008 Lyon, France
[2] Fac RTH Laennec, GENALYS, F-69008 Lyon, France
[3] McGill Univ, Sch Dietet & Human Nutr, Montreal, PQ, Canada
关键词
stable isotopes; mRNA; obesity; sterol-regulatory element-binding protein-1c; fatty-acid synthase; acetyl-CoA carboxylase;
D O I
10.1194/jlr.M200461-JLR200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We assessed the contributions of human liver and adipose tissue de novo lipogenesis (DNL) to triacylglycerol (TAG) synthesis. Volunteers were fed a high-energy, high-carbohydrate diet (HC, n = 5) or a normocaloric diet (NC, n = 10). NC subjects remained in the fasting state (Study 1, n = 5) or received oral glucose (Study 2, n = 5) throughout the test (12 h). HC subjects remained in the fasting state (Study 3). They ingested deuterated water and [U-C-13]acetate to trace lipogenesis. Adipose tissue fatty-acid (FA) synthase (FAS), acetyl-CoA carboxylase 1 (ACC1), and SREBP-1c mRNA were measured. Plasma TAG-FA was labeled by C-13 and deuterium showing active liver lipogenesis, which was stimulated (P < 0.05) by oral glucose and HC diet. Adipose tissue TAG had no detectable C-13 enrichment in any test, showing no significant incorporation of TAG-FA provided by liver lipogenesis, but were labeled by deuterium in all tests, showing active DNL in situ; however, rough quantitative estimates showed that adipose DNL was minimal (<1 g), and poorly stimulated by oral glucose or HC diet. mRNA levels were not increased by the HC diet.jlr Adipose DNL is active in humans, but contributes little to TAG stores and is less responsive than liver DNL to stimulation by carbohydrates.
引用
收藏
页码:846 / 853
页数:8
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