Synergistic control of cellular adhesion by transmembrane 9 proteins

被引:49
作者
Benghezal, M [1 ]
Cornillon, S
Gebbie, L
Alibaud, L
Brückert, F
Letourneur, F
Cosson, P
机构
[1] Univ Geneva, Ctr Med Univ Geneva, Dept Morphol, CH-1211 Geneva, Switzerland
[2] CEA, CNRS, UMR 314, Lab Biochim & Biophys Syst Integres, Grenoble, France
[3] Univ Lyon 1, CNRS, UMR 5086, Inst Biol & Chim Prot, F-69367 Lyon, France
关键词
D O I
10.1091/mbc.E02-11-0724
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
The transmembrane 9 (TM9) family of proteins contains numerous members in eukaryotes. Although their function remains essentially unknown in higher eukaryotes, the Dictyostelium discoideum Phg1a TM9 protein was recently reported to be essential for cellular adhesion and phagocytosis. Herein, the function of Phg1a and of a new divergent member of the TM9 family called Phg1b was further investigated in D. discoideum. The phenotypes of PHG1a, PHG1b, and PHG1a/PHG1b double knockout cells revealed that Phg1a and Phg1b proteins play a synergistic but not redundant role in cellular adhesion, phagocytosis, growth, and development. Complementation analysis supports a synergistic regulatory function rather than a receptor role for Phg1a and Phg1b proteins. Together, these results suggest that Phg1 proteins act as regulators of cellular adhesion, possibly by controlling the intracellular transport in the endocytic pathway and the composition of the cell surface.
引用
收藏
页码:2890 / 2899
页数:10
相关论文
共 13 条
[11]   The iodocyanopindolol and SM-11044 binding protein belongs to the TM9SF multispanning membrane protein superfamily [J].
Sugasawa, T ;
Lenzen, G ;
Simon, S ;
Hidaka, J ;
Cahen, A ;
Guillaume, JL ;
Camoin, L ;
Strosberg, AD ;
Nahmias, C .
GENE, 2001, 273 (02) :227-237
[12]  
SUSSMAN M, 1987, METHOD CELL BIOL, V28, P9
[13]  
VOGEL G, 1980, J CELL BIOL, V86, P456