Murine long-term repopulating ability is compromised by ex vivo culture in serum-free medium despite preservation of committed progenitors

被引:17
作者
Sekhar, M [1 ]
Yu, JM [1 ]
Soma, T [1 ]
Dunbar, CE [1 ]
机构
[1] NHLBI, Hematol Branch, NIH, Bethesda, MD 20892 USA
来源
JOURNAL OF HEMATOTHERAPY | 1997年 / 6卷 / 06期
关键词
D O I
10.1089/scd.1.1997.6.543
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Hematopoietic progenitor cells can be maintained and expanded ex vivo in standard or serum-free culture medium supplemented with a variety of stimulatory cytokines, The use of serum-free medium allows specification of reproducible and precise growth conditions optimal for various applications and is more acceptable from a safety and regulatory point of view, Human and murine committed progenitor cells have been shown to be equivalently or better supported by serum-free culture conditions in the presence of multicytokine combinations, but there is little information on the effects of such culture conditions on repopulating stem cells, We used a murine competitive repopulation model to assess the effect of serum-free versus serum-containing ex vivo culture on long-term reconstituting cells, Despite equivalent numbers of committed CFU-C and day 12 CFU-S present after 4 days of culture of murine marrow in serum-free or serum-containing conditions in the presence of IL-3, IL-6, and SCF, long-term reconstituting activity was significantly impaired by serum-free culture, These findings may have important implications for transplantation and gene therapy applications.
引用
收藏
页码:543 / 549
页数:7
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