Mitochondrial permeability transition in apoptosis and necrosis

被引:128
作者
Hirsch, T [1 ]
Susin, SA [1 ]
Marzo, I [1 ]
Marchetti, P [1 ]
Zamzami, N [1 ]
Kroemer, G [1 ]
机构
[1] CNRS, Unite Propre Rech 420, Villejuif, France
关键词
mitochondrial transmembrane potential; permeability transition; programmed cell death; proteases;
D O I
10.1023/A:1007486022411
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Apoptosis has classically been viewed as a process not involving mitochondria, whereas the implication of mitochondrial dysfunction in necrosis has been recognized for several decades. Recently, it has become clear that apoptosis implies a disruption of mitochondrial membrane intregrity that is decisive for the cell death process. Cytofluorometric methods assessing the mitochondrial membrane function and structure can be employed to demonstrate that, at least in most models of apoptosis, mitochondrial changes precede caspase and nuclease activation. Moreover, pharmacological and genetic experiments suggest that the loss of mitochondrial membrane integrity is a critical event of the apoptotic process, beyond or at the point of no return of programmed cell death. Inhibitors of the mitochondrial megachannel (= permeability transition pore) can prevent both the mitochondrial and the post-mitochondrial manifestations of apoptosis.
引用
收藏
页码:141 / 145
页数:5
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