Involvement of an FtsH homologue in the assembly of functional photosystem I in the cyanobacterium Synechocystis sp PCC 6803

被引:86
作者
Mann, NH [1 ]
Novac, N
Mullineaux, CW
Newman, J
Bailey, S
Robinson, C
机构
[1] Univ Warwick, Dept Biol Sci, Coventry CV4 7AL, W Midlands, England
[2] UCL, Dept Biol, London WC1E 6BT, England
来源
FEBS LETTERS | 2000年 / 479卷 / 1-2期
关键词
AAA protease; cyanobacterium; photosynthesis; photosystem I; Synechocystis;
D O I
10.1016/S0014-5793(00)01871-8
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Synechocystis sp, PCC 6803 genome encodes four putative homologues of the AAA protease FtsH, two of which (slr0228 and sll1463) have been subjected to insertional mutagenesis in this study. Disruption of sll1463 had no discernible effect but disruption of slr0228 caused a 60%, reduction in the abundance of functional photosystem I, without affecting the cellular content of photosystem II or phycobilisomes, Fluorescence and immunoblotting analyses show reductions in PS I polypeptides and possible structural alterations in the residual PS I, indicating an important role for slr0228 in PS I biogenesis, (C) 2000 Federation of European Biochemical Societies, Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:72 / 77
页数:6
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