Use of FLP/FRT system to study Drosophila development

被引：110

作者：

Theodosiou, NA ^{[1
]}

Xu, T ^{[1
]}

机构：

[1] Yale Univ, Sch Med, Dept Genet, Boyer Ctr Mol Med,Howard Hughes Med Inst, New Haven, CT 06510 USA

来源：

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY | 1998年 / 14卷 / 04期

关键词：

D O I：

10.1006/meth.1998.0591

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Marked clones in mosaic animals have been used extensively to answer developmental questions in Drosophila. Recently, the use of the FLP/FRT system has allowed for the high-frequency production of mosaic animals for 95% of Drosophila genes. Cell markers have been engineered in this system to label mutant clones in both developing tissues and adult cuticle. Strains carrying these genetically marked FLP/FRT chromosomes have greatly enhanced our ability to study gene function in both germline and somatic Drosophila cells. (C) 1998 Academic Press.

引用

页码：355 / 365

页数：11

共 27 条

[1]

Ashburner M., 1989, DROSOPHILA LAB HDB

[2] COMPARTMENT BOUNDARIES AND THE CONTROL OF DROSOPHILA LIMB PATTERN BY HEDGEHOG PROTEIN [J].

BASLER, K ;

STRUHL, G .

NATURE, 1994, 368 (6468) :208-214

[3]

BECKER, 1976, DEV BIOL, V48, P1

[4] P-ELEMENT-MEDIATED ENHANCER DETECTION - A VERSATILE METHOD TO STUDY DEVELOPMENT IN DROSOPHILA [J].

BELLEN, HJ ;

OKANE, CJ ;

WILSON, C ;

GROSSNIKLAUS, U ;

PEARSON, RK ;

GEHRING, WJ .

GENES & DEVELOPMENT, 1989, 3 (09) :1288-1300

[5]

BUSSON D, 1983, GENETICS, V105, P309

[6]

CHOU TB, 1992, GENETICS, V131, P643

[7]

Chou TB, 1996, GENETICS, V144, P1673

[8] MAMMALIAN CD2 IS AN EFFECTIVE HETEROLOGOUS MARKER OF THE CELL-SURFACE IN DROSOPHILA [J].

DUNINBORKOWSKI, OM ;

BROWN, NH .

DEVELOPMENTAL BIOLOGY, 1995, 168 (02) :689-693

[9] ISOLATION OF MONOCLONAL-ANTIBODIES SPECIFIC FOR HUMAN C-MYC PROTO-ONCOGENE PRODUCT [J].

EVAN, GI ;

LEWIS, GK ;

RAMSAY, G ;

BISHOP, JM .

MOLECULAR AND CELLULAR BIOLOGY, 1985, 5 (12) :3610-3616

[10] INDUCTION, DETECTION AND CHARACTERIZATION OF CELL-DIFFERENTIATION MUTANTS IN DROSOPHILA [J].

GARCIABELLIDO, A ;

DAPENA, J .

MOLECULAR AND GENERAL GENETICS, 1974, 128 (02) :117-130

← 1 2 3 →