Alteration of redox state of human serum albumin in patients under anesthesia and invasive surgery

Human serum albumin is a mixture of mercapt-(HMA, reduced form) and nonmercaptalbumin (HNA, oxidized form). We studied the mercapt <----> nonmercapt conversion of human serum albumin, which reflects the redox state of the extracellular fluids, in cardiac and other common surgical patients using high-performance liquid chromatography. Mean values of [(HMA)/(HMA+HNA)]+/-standard deviation, [(f) over bar(HMA)+/-sigma], for patients who received common surgery (group 1) and cardiac surgery (group 2) at the start of anesthesia were 0.636+/-0.050 (n=83) and 0.615+/-0.062 (n=14), respectively. (f) over bar(HMA) values were markedly lower than those for healthy male adults of 0.750+/-0.028 (n=28). (f) over bar(HMA) increased at 24 h after the start of anesthesia and decreased on the 4th postoperative day in most of the patients. These postoperative changes were prominent in cardiac surgical patients. Although (f) over bar(HMA) values after the 7th postoperative day recovered to those at the start of anesthesia in almost all of common surgical patients, those in cardiac surgical patients never recovered even on the 21st postoperative day. (C) 1997 Elsevier Science B.V.