The formation of nucleic acids/8-hydroxy-quinoline/yttrium(III) ternary complexes and their fluorescent properties have been studied. The nucleic acids studied include native and thermally denatured calf thymus DNA, fish sperm DNA and yeast RNA. In the range of pH 7.6-8.5, controlled by NH3-NH4Cl buffer, ternary complexes are formed that fluoresce at different wavelengths with different nucleic acids. Based on the fluorescence reactions, sensitive spectro-fluorimetric methods for nucleic acids are proposed. In optimal conditions, the calibration curves were linear in the range 0.5-4.0 mu g ml(-1) for calf thymus DNA, 0.5-2.5 mu g ml(-1) for fish sperm DNA and 0.5-4.0 mu g ml(-1) for yeast RNA. The limits of determination (3 sigma) were 0.030 mu g ml(-1) for calf thymus DNA, 0.020 mu g ml(-1) for fish sperm DNA and 0.090 mu g ml(-1) for yeast RNA. Corresponding to the interferences of coexisting substances, six synthetic samples were constructed and the results of determination were satisfactory.
机构:
IMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLANDIMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLAND
RAO, J
;
OTTO, WR
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IMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLANDIMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLAND
机构:
IMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLANDIMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLAND
RAO, J
;
OTTO, WR
论文数: 0引用数: 0
h-index: 0
机构:
IMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLANDIMPERIAL CANC RES FUND,HISTOPATHOL UNIT,35-43 LINCOLNS INN FIELDS,LONDON WC2A 3PN,ENGLAND