Spectrofluorimetric determination of nucleic acids as 8-hydroxyquinoline/yttrium ternary complexes

被引:11
作者
Huang, CZ [1 ]
Li, KA [1 ]
Tong, SY [1 ]
机构
[1] BEIJING UNIV,FAC CHEM & MOL ENGN,INST ANALYT CHEM,BEIJING 100871,PEOPLES R CHINA
关键词
spectrofluorimetry; nucleic acids; DNA; RNA; 8-hydroxyquinoline; yttrium;
D O I
10.1007/BF01242326
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The formation of nucleic acids/8-hydroxy-quinoline/yttrium(III) ternary complexes and their fluorescent properties have been studied. The nucleic acids studied include native and thermally denatured calf thymus DNA, fish sperm DNA and yeast RNA. In the range of pH 7.6-8.5, controlled by NH3-NH4Cl buffer, ternary complexes are formed that fluoresce at different wavelengths with different nucleic acids. Based on the fluorescence reactions, sensitive spectro-fluorimetric methods for nucleic acids are proposed. In optimal conditions, the calibration curves were linear in the range 0.5-4.0 mu g ml(-1) for calf thymus DNA, 0.5-2.5 mu g ml(-1) for fish sperm DNA and 0.5-4.0 mu g ml(-1) for yeast RNA. The limits of determination (3 sigma) were 0.030 mu g ml(-1) for calf thymus DNA, 0.020 mu g ml(-1) for fish sperm DNA and 0.090 mu g ml(-1) for yeast RNA. Corresponding to the interferences of coexisting substances, six synthetic samples were constructed and the results of determination were satisfactory.
引用
收藏
页码:231 / 236
页数:6
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