The high-sensitivity determination of protein concentrations by the enhancement of Rayleigh light scattering of Arsenazo-DBN

被引：27

作者：

Li, QF ^{[1
]}

Chen, XG ^{[1
]}

Zhang, HY ^{[1
]}

Xue, CX ^{[1
]}

Liu, SH ^{[1
]}

Hu, Z ^{[1
]}

机构：

[1] Lanzhou Univ, Dept Chem, Lanzhou 730000, Peoples R China

来源：

ANALYST | 2000年 / 125卷 / 08期

关键词：

D O I：

10.1039/b003154m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new Rayleigh light scattering (RLS) assay of protein is presented in this paper. At the optimum pH 4.10, the weak RLS of Arsenazo-DBN can be greatly enhanced by the addition of proteins due to the interaction between protein and Arsenazo-DBN. Based on this, the reactions of Arsenazo-DBN and proteins, including bovine serum albumin, human serum album, gamma-globulin, egg albumin, lysozyme and trypsin, were studied. A new quantitative determination method for proteins has been developed. The linear range for human serum albumin, for example, is 0.085-34.62 mu g mL(-1) with a detection limit of 44.8 ng mL(-1). Besides high sensitivity, the method is characterized by good reproducibility, rapidity of reaction, good stability, and few interfering substances. The determination of the proteins in human serum and urine samples by this method give results very close to those obtained using Coomassie Brilliant Blue G-250 colorimetry, with relative standard deviations of 0.7-2.5%.

引用

页码：1483 / 1486

页数：4

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