The two-component signal system in rice (Oryza sativa L.):: A genome-wide study of cytokinin signal perception and transduction

被引：101

作者：

Du, Liming

Jiao, Fangchan

Chu, Jun

Jin, Gulei

Chen, Ming

Wu, Ping

机构：

[1] Zhejiang Univ, Coll Life Sci, Dept Bioinformat, Hangzhou 310058, Peoples R China

[2] Zhejiang Univ, Coll Life Sci, Key State Lab Plant Physiol & Biochem, Hangzhou 310058, Peoples R China

[3] Zhejiang Univ, Inst Bioinformat, Hangzhou 310029, Peoples R China

来源：

GENOMICS | 2007年 / 89卷 / 06期

基金：

中国国家自然科学基金;

关键词：

cytokinin two-component system; Rice; histidine kinase; histidine phosphotransfer proteins; response regulator; genomic analysis; phylogenetic analysis; yeast experiment; RT-PCR;

D O I：

10.1016/j.ygeno.2007.02.001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In this report we define the genes of two-component regulatory systems in rice through a comprehensive computational analysis of rice (Oryza sativa L.) genome sequence databases. Thirty-seven genes were identified, including 5 HKs (cytokinin-response histidine protein kinase) (OSHK1-4, OSHKL1), 5 HPs (histidine phosphotransfer proteins) (OsHP1-5), 15 type-A RRs (response regulators) (OsRR1-15), 7 type B RR genes (OsRR16-22), and 5 predicted pseudo-response regulators (OsPRR1-5). Protein motif organization, gene structure, phylogenetic analysis, chromosomal location, and comparative analysis between rice, maize, and Arabidopsis are described. Full-length cDNA clones of each gene were isolated from rice. Heterologous expression of each of the OsHKs in yeast mutants conferred histidine kinase function in a cytokinin-dependent manner. Nonconserved regions of individual cDNAs were used as probes in expression profiling experiments. This work provides a foundation for future functional dissection of the rice cytokinin two-component signaling pathway. (c) 2007 Elsevier Inc. All rights reserved.

引用

页码：697 / 707

页数：11