Quantitative demonstration of intrathecal synthesis of high affinity immunoglobulin G in herpes simplex encephalitis using affinity-mediated immunoblotting

被引：3

作者：

Chapman, Miles D. ^{[1
]}

Thompson, Edward J. ^{[1
]}

Candler, Paul M. ^{[1
]}

Dale, Russell C. ^{[1
]}

Church, Andrew J. ^{[1
]}

Giovannoni, Gavin ^{[1
]}

机构：

[1] Inst Neurol, Dept Neuroimmunol, London WC1N 3BG, England

来源：

JOURNAL OF NEUROIMMUNOLOGY | 2007年 / 185卷 / 1-2期

关键词：

affinity; IgG; herpes simplex encephalitis; thiocyanate;

D O I：

10.1016/j.jneuroim.2007.01.002

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Three paired serial samples of CSF and serum (from days 8, 13 and 22) were taken from a patient referred to the National Hospital for Neurology and Neurosurgery with what was duly confirmed as having herpes simplex encephalitis using PCR. The samples were investigated using affinity-mediated immunoblotting followed by incubation with sodium thiocyanate. Digitisation of the blots enabled further analysis. We showed that the clones of antigen-specific IgG, which were produced intrathecally, were of higher relative affinity than polyclonal antigen-specific IgG. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：130 / 135

页数：6

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