Localization of NADPH-protochlorophyllide reductase in plastids of barley at different greening stages

被引：25

作者：

Barthélemy, X ^{[1
]}

Bouvier, G

Radunz, A

Docquier, S

Schmid, GH

Franck, F

机构：

[1] Univ Liege, Inst Plant Biol B22, Lab Photobiol, B-4000 Liege, Belgium

[2] Univ Bielefeld, Fak Biol, Lehrstuhl Zellphysiol, D-4800 Bielefeld 1, Germany

[3] Univ Liege, Inst Plant Biol B22, Lab Plant Morphol, B-4000 Liege, Belgium

来源：

PHOTOSYNTHESIS RESEARCH | 2000年 / 64卷 / 01期

关键词：

chlorophyll synthesis; chloroplast; envelope membrane; greening; NADPH-protochlorophyllide oxidoreductase; protochlorophyllide; thylakoid;

D O I：

10.1023/A:1026576319029

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The localization of protochorophyllide (Pchlide) and of NADPH-protochlorophyllide oxidoreductase (POR, EC 1.6.99.1) within (etio)chloroplasts has been investigated at selected stages of greening of barley seedlings. Pchlide pigment and POR protein contents were evaluated in different plastid membrane fractions by fluorescence spectroscopy and immunoblot analysis using a monospecific polyclonal antibody raised against the purified enzyme. Fluorescence analysis showed the presence of Pchlide in both the envelope and thylakoid membranes. During greening, the Pchlide content, expressed on a total protein basis, decreased in thylakoid membranes, whereas it increased in the envelope membranes. POR proteins were detected mainly in thylakoid membranes at early greening stages. In contrast, the weak amount of POR proteins was associated more specifically with envelope membranes of mature chloroplasts. Whatever the greening stage, thylakoid-bound Pchlide and POR proteins were more abundant in the thylakoid regions which remained unsolubilized after mild Triton treatment used as standard procedure to prepare PS II particles. This suggests the preferential association of Pchlide and POR to the appressed regions of thylakoids.

引用

页码：63 / 76

页数：14

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