DNA target sequence and FNR-dependent gene expression

被引：26

作者：

Scott, C ^{[1
]}

Partridge, JD ^{[1
]}

Stephenson, JR ^{[1
]}

Green, J ^{[1
]}

机构：

[1] Univ Sheffield, Krebs Inst Biomol Res, Dept Mol Biol & Biotechnol, Western Bank, Sheffield S10 2TN, S Yorkshire, England

来源：

FEBS LETTERS | 2003年 / 541卷 / 1-3期

基金：

英国生物技术与生命科学研究理事会;

关键词：

cAMP receptor protein-FNR family; oxygen sensing; transcriptional regulation;

D O I：

10.1016/S0014-5793(03)00312-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

FNR proteins are global transcription regulators that respond to fluctuations in environmental oxygen. They recognise a DNA target consisting of an inverted repeat, TTGA-TN(1)N(2)N(3)N(4)ATCAA (where N1-4 represents a non-conserved tetrad, NCT). Analysis of 68 known and predicted FNR sites from the Escherichia coli K12 genome revealed a bias toward A or T at positions N-2 and N-3 of the NCT. The effect of the NCT sequence on FNR-dependent transcription in vivo was assessed using a series of class II and class I model promoters with different NCT sequences. Changing the NCT sequence did not affect basal activity but altered anaerobic induction by as much as an order of magnitude. Thus, the NCT sequence is a fundamental component in setting the dynamic range of the FNR switch. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：97 / 101

页数：5

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