Protein-Nanoparticle Interaction: Identification of the Ubiquitin-Gold Nanoparticle Interaction Site

被引：227

作者：

Calzolai, Luigi ^{[1
]}

Franchini, Fabio ^{[1
]}

Gilliland, Douglas ^{[1
]}

Rossi, Francois ^{[1
]}

机构：

[1] Commiss European Communities, Joint Res Ctr, Inst Hlth & Consumer Protect, I-21027 Ispra, Varese, Italy

来源：

NANO LETTERS | 2010年 / 10卷 / 08期

关键词：

Gold nanoparticles; NMR; protein-nanoparticle interactions; chemical shift mapping; NMR; SURFACE; SIZE;

D O I：

10.1021/nl101746v

中图分类号：

O6 [化学];

学科分类号：

070301 [无机化学];

摘要：

We demonstrate that it is possible to identify the protein-nanoparticle interaction site at amino acid scale in solution. Using NMR, chemical shift perturbation analysis, and dynamic light scattering we have identified a specific domain of human ubiquitin that interacts with gold nanoparticles. This method allows a detailed structural analysis of proteins absorbed onto surfaces of nanoparticles in physiological conditions and it will provide much needed experimental data for better modeling and prediction of protein-nanoparticle interactions.

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收藏

页码：3101 / 3105

页数：5

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