The phrA gene of Rhodobacter sphaeroides encodes a photolyase and is regulated by singlet oxygen and peroxide in a σE-dependent manner

被引：31

作者：

Hendrischk, Anne-Kathrin ^{[1
]}

Braatsch, Stephan ^{[1
]}

Glaeser, Jens ^{[1
]}

Klug, Gabriele ^{[1
]}

机构：

[1] Univ Giessen, Inst Mikrobiol & Mol Biol, D-35392 Giessen, Germany

来源：

MICROBIOLOGY-SGM | 2007年 / 153卷

关键词：

D O I：

10.1099/mic.0.2006/004390-0

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The genome of the facultatively photosynthetic bacterium Rhodobacter sphaeroides encodes three proteins of the photolyase/cryptochrome family. This paper shows that phrA (RSP2143) encodes a functional photolyase, which is an enzyme that repairs UV radiation-induced DNA damage in a blue light dependent manner. Expression of phrA is upregulated in response to light, with no photoreceptor or the photosynthetic electron transport being involved. The results reveal that singlet oxygen and hydrogen peroxide dependent signals are transmitted by the sigma(E) factor and the anti-sigma(E) factor ChrR affecting phrA expression, while superoxide anions do not stimulate phrA expression. Thus, the sigma(E) regulon is involved not only in the response to singlet oxygen but also in the hydrogen peroxide response.

引用

页码：1842 / 1851

页数：10

共 53 条

[41] A BROAD HOST RANGE MOBILIZATION SYSTEM FOR INVIVO GENETIC-ENGINEERING - TRANSPOSON MUTAGENESIS IN GRAM-NEGATIVE BACTERIA [J].

SIMON, R ;

PRIEFER, U ;

PUHLER, A .

BIO-TECHNOLOGY, 1983, 1 (09) :784-791

[42] CONTROL OF PHOTOSYNTHETIC MEMBRANE ASSEMBLY IN RHODOBACTER-SPHAEROIDES MEDIATED BY PUHA AND FLANKING SEQUENCES [J].

SOCKETT, RE ;

DONOHUE, TJ ;

VARGA, AR ;

KAPLAN, S .

JOURNAL OF BACTERIOLOGY, 1989, 171 (01) :436-446

[43] Crystal structure of DNA photolyase from Anacystis nidulans [J].

Tamada, T ;

Kitadokoro, K ;

Higuchi, Y ;

Inaka, K ;

Yasui, A ;

deRuiter, PE ;

Eker, APM ;

Miki, K .

NATURE STRUCTURAL BIOLOGY, 1997, 4 (11) :887-891

[44] Effects of photosynthetic reaction center H protein domain mutations on photosynthetic properties and reaction center assembly in Rhodobacter sphaeroides [J].

Tehrani, A ;

Prince, RC ;

Beatty, JT .

BIOCHEMISTRY, 2003, 42 (30) :8919-8928

[45] An inducible tellurite-resistance operon in Proteus mirabilis [J].

Toptchieva, A ;

Sisson, G ;

Bryden, LJ ;

Taylor, DE ;

Hoffman, PS .

MICROBIOLOGY-SGM, 2003, 149 :1285-1295

[46] Identification and characterization of a second chromophore of DNA photolyase from Thermus thermophilus HB27 [J].

Ueda, T ;

Kato, A ;

Kuramitsu, S ;

Terasawa, H ;

Shimada, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (43) :36237-36243

[47]

van Niel CB, 1944, BACTERIOL REV, V8, P1

[48] THE PUC PLASMIDS, AN M13MP7-DERIVED SYSTEM FOR INSERTION MUTAGENESIS AND SEQUENCING WITH SYNTHETIC UNIVERSAL PRIMERS [J].

VIEIRA, J ;

MESSING, J .

GENE, 1982, 19 (03) :259-268

[49] The signaling mechanism of Arabidopsis CRY1 involves direct interaction with COP1 [J].

Yang, HQ ;

Tang, RH ;

Cashmore, AR .

PLANT CELL, 2001, 13 (12) :2573-2587

[50] ENHANCEMENT OF PHOTOREPAIR OF ULTRAVIOLET-DAMAGE BY PREILLUMINATION WITH FLUORESCENT LIGHT IN CULTURED FISH CELLS [J].

YASUHIRA, S ;

MITANI, H ;

SHIMA, A .

PHOTOCHEMISTRY AND PHOTOBIOLOGY, 1991, 53 (02) :211-215

← 1 2 3 4 5 6 →