Differential localization of ADAM1a and ADAM1b in the endoplasmic reticulum of testicular germ cells and on the surface of epididymal sperm

被引：47

作者：

Kim, E ^{[1
]}

Nishimura, H ^{[1
]}

Baba, T ^{[1
]}

机构：

[1] Univ Tsukuba, Inst Appl Biochem, Tsukuba, Ibaraki 3058572, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 304卷 / 02期

基金：

日本学术振兴会;

关键词：

ADAM; spermatogenesis; fertilization; sperm-egg binding; sperm-egg fusion; germ cells; endoplasmic reticulum;

D O I：

10.1016/S0006-291X(03)00588-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Although fertilin is a heterodimeric complex between ADAM1 (A Disintegrin And Metalloprotease 1, fertilin alpha) and ADAM2 (fertilin beta) located on the sperm surface, two different ADAM1 isoforms, ADAM1a and ADAM1b, are present in the mouse testis. In this study, we have examined the localization of ADAM1a and ADAM1b in testicular germ cells and epididymal sperm. ADAM1a was restrictedly present within the endoplasmic reticulum of germ cells, whereas epididymal sperm contained only ADAM1b on the cell surface. The precursors of ADAM1a and ADAM1b formed a heterodimeric complex with that of ADAM2 in the endoplasmic reticulum, of germ cells. The heterodimeric complex between the mature forms of ADAM1b and ADAM2 was also found on the sperm surface. These data imply the potential roles of ADAM1a and ADAM1b in spermatogenesis and fertilization, respectively. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：313 / 319

页数：7

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