The temperature-sensitive ion channel TRPV2 is endogenously expressed and functional in the primary sensory cell line F-11

被引:31
作者
Bender, FLP
Schnitzler, MMY
Li, YZ
Ji, AL
Weihe, E
Gudermann, T
Schäfer, MKH
机构
[1] Univ Marburg, Inst Anat & Cell Biol, D-35032 Marburg, Germany
[2] Univ Marburg, Inst Pharmacol & Toxicol, D-35032 Marburg, Germany
关键词
nociception; temperature; vanilloid receptor; noxious heat; ruthenium red; dorsal root ganglion;
D O I
10.1159/000083651
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
In sensory neurons heat is transduced by a subfamily of TRP channels sharing sequence homology with the capsaicin- sensitive vanilloid receptor subtype 1 ( TRPV1), but differing in their thermal response thresholds. To identify a neuronal cell line endogenously expressing noxious heat- transducing ion channels, we examined F- 11 cells, a hybridoma derived from rat dorsal root ganglia and mouse neuroblastoma. Using RT- PCR, transcripts homologous to TRPV2 and TRPV4, but not to TRPV1 or TRPV3, were found. We isolated a full- length cDNA of 2.4 kb coding for a 757- amino acid protein corresponding to mouse TRPV2, which was further characterized by immunocytochemistry and electrophysiology. Using the whole- cell patch- clamp technique, we observed a heat- evoked increase in outward and inward currents with a threshold of 51.6 +/- 0.2degreesC. The current- voltage relationship stimulated by a temperature of 52degreesC was characterized by an outward rectification with a reversal potential close to - 10 mV. Heat- evoked currents could be inhibited by ruthenium red. There was no activation by stimulation with capsaicin or 2- aminoethoxydiphenyl borate. Our results indicate that F- 11 cells express functional noxious heat- sensitive TRPV2 channels. Thus, we propose that F- 11 cells represent a valuable in vitro model to characterize the properties of TRPV2 in a native neuronal environment. Copyright (C) 2005 S. Karger AG, Basel.
引用
收藏
页码:183 / 194
页数:12
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