Bioconversion of fumaric acid to succinic acid by recombinant E-coli

被引：30

作者：

Wang, XH ^{[1
]}

Gong, CS ^{[1
]}

Tsao, GT ^{[1
]}

机构：

[1] Purdue Univ, Renewable Resources Engn Lab, Potter Engn Ctr 1295, W Lafayette, IN 47907 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1998年 / 70-2卷 / 1期

关键词：

recombinant E-coli; fumarate reductase; succinic acid; fumaric acid;

D O I：

10.1007/BF02920202

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Succinic acid was produced efficiently from fumaric acid by a recombinant E. coli strain DH5 alpha/pGC1002 containing multicopy fumarate reductase genes. The effects of initial fumaric acid and glucose concentration on the production of succinic acid were investigated. Succinic acid reached 41 to over 60 g/L in 48.5 h starting with 50 to 64 g/L fumaric acid. Significant substrate inhibition was observed at initial fumaric acid concentration of 90 g/L. L-Malic acid became the major fermentation product under these conditions. Provision of glucose (5-30 g/L) to the fermentation medium stimulated the initial succinic acid production rate over two folds.

引用

页码：919 / 928

页数：10