Monoclonal antibodies generated against recombinant ATM support kinase activity

被引：7

作者：

Alligood, KJ

Milla, M

Rhodes, N

Ellis, B

Kilpatrick, KE

Lee, A

Gilmer, TM

Lansing, TJ

机构：

[1] Glaxo Wellcome Inc, Res & Dev, Dept Canc Biol, Res Triangle Pk, NC 27709 USA

[2] Glaxo Wellcome Inc, Res & Dev, Dept Prot Sci, Res Triangle Pk, NC 27709 USA

来源：

HYBRIDOMA | 2000年 / 19卷 / 04期

关键词：

D O I：

10.1089/027245700429864

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report on the rapid generation of two monoclonal antibodies, ATM A16.35 and ATM. D16.11, that bind to the kinase domain of mutated ataxia telangiectasia (ATM). These antibodies were generated against E. coli-expressed recombinant protein using the RIMMS strategy. We show that ATM A16.35 binds ATM by Western blot analysis, and ATM D16.11 forms immune complexes with native ATM in immunoprecipitations without neutralizing kinase activity.

引用

页码：317 / 321

页数：5

共 16 条

[11] Identification of tyrosine phosphorylated adhesion proteins in human cancer cells
Kinch, MS
Kilpatrick, KE
Zhong, CL
[J]. HYBRIDOMA, 1998, 17 (03): : 227 - 235
[12] The genetic defect in ataxia-telangiectasia
Lavin, MF
Shiloh, Y
[J]. ANNUAL REVIEW OF IMMUNOLOGY, 1997, 15 : 177 - 202
[13] Production of monoclonal antibodies using recombinant baculovirus displaying gp64-fusion proteins
Lindley, KM
Su, JL
Hodges, PK
Wisely, GB
Bledsoe, RK
Condreay, JP
Winegar, DA
Hutchins, JT
Kost, TA
[J]. JOURNAL OF IMMUNOLOGICAL METHODS, 2000, 234 (1-2) : 123 - 135
[14] Sarkaria JN, 1998, CANCER RES, V58, P4375
[15] Shorter development of immunoassay for drugs: application of the novel RIMMS technique enables rapid production of monoclonal antibodies to ranitidine
Wring, SA
Kilpatrick, KE
Hutchins, JT
Witherspoon, SM
Ellis, B
Jenner, WN
Serabjit-Singh, C
[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1999, 19 (05) : 695 - 707
[16] WRING SA, 1998, METHODOLOGICAL SURVE, V25, P181

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