Optimisation of PCR reactions using primer chessboarding

被引：18

作者：

Gunson, R ^{[1
]}

Gillespie, G ^{[1
]}

Carman, WF ^{[1
]}

机构：

[1] Gartnavel Royal Hosp, W Scotland Specialist Virol Ctr, Glasgow G12 OYN, Lanark, Scotland

来源：

JOURNAL OF CLINICAL VIROLOGY | 2003年 / 26卷 / 03期

关键词：

polymerase chain reaction; reagent; primer;

D O I：

10.1016/S1386-6532(03)00006-4

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In-house polymerase chain reaction (PCR) assays are now an integral part of the work of most diagnostic microbiological laboratories. Despite the availability of commercial reagent 'master-mixes' of some PCR reagents, the optimisation of primers still poses a significant problem. Here, we describe a simple method to assess the concentration of primer needed in single round, multiplex, nested and 'real-time' PCR procedures. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：369 / 373

页数：5