Localization and topology of ratp28, a member of a novel family of putative steroid-binding proteins

被引：44

作者：

Nölte, I ^{[1
]}

Jeckel, D ^{[1
]}

Wieland, FT ^{[1
]}

Sohn, K ^{[1
]}

机构：

[1] Univ Heidelberg, BZH, D-69120 Heidelberg, Germany

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 2000年 / 1543卷 / 01期

关键词：

steroid-binding protein; endoplasmic reticulum; type II topology;

D O I：

10.1016/S0167-4838(00)00188-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have cloned ratp28, a membrane protein from rat liver homologous to the previously described hpr6.6, a putative steroid-binding protein in humans. Ratp28 has a type II topology as determined by protease digestion experiments on intact and detergent-solubilized membranes. Subcellular fractionation by sucrose density centrifugation revealed a distribution for ratp28 identical to Bip as a marker for membranes of the endoplasmic reticulum. In these experiments no association was found with markers for Golgi or plasma membranes, indicating that ratp28 is localized to the endoplasmic reticulum. (C) 2000 Elsevier Science B.V. All rights reserved.

引用

页码：123 / 130

页数：8

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