The human VASA gene is specifically expressed in the germ cell lineage

被引：469

作者：

Castrillon, DH

Quade, BJ

Wang, TY

Quigley, C

Crum, CP

机构：

[1] Brigham & Womens Hosp, Dept Pathol, Womens & Perinatal Pathol Div, Boston, MA 02115 USA

[2] Mackay Mem Hosp, Dept Obstet & Gynecol, Taipei, Taiwan

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2000年 / 97卷 / 17期

关键词：

gametogenesis; DEAD box RNA helicase;

D O I：

10.1073/pnas.160274797

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

To understand the origins and function of the human germ cell lineage and to identify germ cell-specific markers we have isolated a human ortholog of the Drosophila gene vase, The gene was mapped to human chromosome 5q (near the centromere) by radiation hybrid mapping. We show by Northern analysis of fetal and adult tissues that expression of the human VASA gene is restricted to the ovary and testis and is undetectable in somatic tissues. We generated polyclonal antibodies that bind to the VASA protein in formalin-fixed, paraffin-embedded tissue and characterized VASA protein expression in human germ cells at various stages of development. The VASA protein is cytoplasmic and expressed in migratory primordial germ cells in the region of the gonadal ridge. VASA protein is present in fetal and adult gonadal germ cells in both males and females and is most abundant in spermatocytes and mature oocytes, The gene we have isolated is thus a highly specific marker of germ cells and should be useful for studies of human germ cell determination and function.

引用

页码：9585 / 9590

页数：6

共 33 条

[11] Microtubule-dependent recruitment of Staufen-Green fluorescent protein into large RNA-containing granules and subsequent dendritic transport in living hippocampal neurons [J].