Cloning and chromosomal mapping of an orphan chemokine receptor: mouse RDC1

被引：55

作者：

Heesen, M

Berman, MA

Charest, A

Housman, D

Gerard, C

Dorf, ME

机构：

[1] Harvard Univ, Sch Med, Dept Pathol, Boston, MA 02115 USA

[2] MIT, Ctr Canc Res, Cambridge, MA 02139 USA

[3] Childrens Hosp, Ina Sue Perlmutter Res Labs, Boston, MA 02115 USA

来源：

IMMUNOGENETICS | 1998年 / 47卷 / 05期

关键词：

astrocytes; receptors; chemokines; mouse chromosome 1;

D O I：

10.1007/s002510050371

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Degenerate RT-PCR was used to identify a new seven-transmembrane-spanning receptor expressed in astrocytes. A receptor, termed RDC1, displaying the characteristic structural features of a chemokine receptor was cloned. The predicted 362-amino-acid sequence displayed 92% and 91% similarity to the human and dog orphan receptor RDC1, respectively. In addition, RDC1 shares 43% amino acid similarity to rabbit and mouse CXCR2. Transcripts of RDC1 were found in astrocytes, heart, kidney, the mesangial tumor line MES-13, spleen, and neutrophils by means of northern blot. Using linkage analysis of interspecies backcross mice, we localized to chromosome 1 the genes for mouse CXCR2, CXCR4, and RDC1. Mouse RDC1 is linked to and lies between the genes for the mouse CXC chemokine receptors CXCR2 and CXCR4. The combined data of chromosomal location and sequence similarity suggest that RDC1 is an orphan CXC chemokine receptor.

引用

收藏

页码：364 / 370

页数：7

相关论文

共 47 条

[1] MOLECULAR EVOLUTION OF THE HUMAN INTERLEUKIN-8 RECEPTOR GENE-CLUSTER [J].

AHUJA, SK ;

OZCELIK, T ;

MILATOVITCH, A ;

FRANCKE, U ;

MURPHY, PM .

NATURE GENETICS, 1992, 2 (01) :31-36

[2] BASIC LOCAL ALIGNMENT SEARCH TOOL [J].

ALTSCHUL, SF ;

GISH, W ;

MILLER, W ;

MYERS, EW ;

LIPMAN, DJ .

JOURNAL OF MOLECULAR BIOLOGY, 1990, 215 (03) :403-410

[3] Identification of CCR6, the specific receptor for a novel lymphocyte-directed CC chemokine LARC [J].

Baba, M ;

Imai, T ;

Nishimura, M ;

Kakizaki, M ;

Takagi, S ;

Hieshima, K ;

Nomiyama, H ;

Yoshie, O .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1997, 272 (23) :14893-14898

[4] CC-CHEMOKINES IN ALLERGIC INFLAMMATION [J].

BAGGIOLINI, M ;

DAHINDEN, CA .

IMMUNOLOGY TODAY, 1994, 15 (03) :127-133

[5] SIGNALS AND RECEPTORS INVOLVED IN RECRUITMENT OF INFLAMMATORY CELLS [J].

BENBARUCH, A ;

MICHIEL, DF ;

OPPENHEIM, JJ .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (20) :11703-11706

[6] Molecular cloning and functional expression of murine JE (Monocyte chemoattractant protein 1) and murine macrophage inflammatory protein la receptors - Evidence for two closely linked C-C chemokine receptors on chromosome 9 [J].

Boring, L ;

Gosling, J ;

Monteclaro, FS ;

Lusis, AJ ;

Tsou, CL ;

Charo, IF .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (13) :7551-7558

[7]

BOZIC CR, 1994, J BIOL CHEM, V269, P29355

[8] THE MURINE HOMOLOGUE OF THE HUMAN INTERLEUKIN-8 RECEPTOR-TYPE-B MAPS NEAR THE ITY-LSH-BCG DISEASE RESISTANCE LOCUS [J].

CERRETTI, DP ;

NELSON, N ;

KOZLOSKY, CJ ;

MORRISSEY, PJ ;

COPELAND, NG ;

GILBERT, DJ ;

JENKINS, NA ;

DOSIK, JK ;

MOCK, BA .

GENOMICS, 1993, 18 (02) :410-413

[9] MOLECULAR-CLONING AND FUNCTIONAL EXPRESSION OF 2 MONOCYTE CHEMOATTRACTANT PROTEIN-1 RECEPTORS REVEALS ALTERNATIVE SPLICING OF THE CARBOXYL-TERMINAL TAILS [J].

CHARO, IF ;

MYERS, SJ ;

HERMAN, A ;

FRANCI, C ;

CONNOLLY, AJ ;

COUGHLIN, SR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (07) :2752-2756

[10] The beta-chemokine receptors CCR3 and CCR5 facilitate infection by primary HIV-1 isolates [J].

Choe, H ;

Farzan, M ;

Sun, Y ;

Sullivan, N ;

Rollins, B ;

Ponath, PD ;

Wu, LJ ;

Mackay, CR ;

LaRosa, G ;

Newman, W ;

Gerard, N ;

Gerard, C ;

Sodroski, J .

CELL, 1996, 85 (07) :1135-1148

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