Multiple S gene family members including natural antisense transcripts are differentially expressed during development of maize flowers

被引:12
作者
Ansaldi, R [1 ]
Chaboud, A [1 ]
Dumas, C [1 ]
机构
[1] Ecole Normale Super Lyon, UCBLyon 1, INRA, CNRS,UMR 5667, F-69634 Lyon 07, France
关键词
D O I
10.1074/jbc.M003047200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Within the large Brassica S gene family, SLG (S locus glycoprotein) and SRK (S locus receptor kinase) participate to the control of pollen-stigma self-incompatibility, In the self-compatible species maize, S gene family members are predominantly expressed in vegetative organs but are also expressed to a lesser extent in the stigma (silk), To determine if the expression of any S gene family members correlates with female receptivity, we analyzed their expression in developing maize silks, We show that a large family of maize S transcripts is expressed in developing silks. Surprisingly, we isolated a cDNA complementary to a large portion of the antisense strand of the maize receptor kinase S domain. Rapid amplification of cDNA ends (RACE)-polymerase chain reaction, RNase protection, and Northern hybridization with single-stranded riboprobes confirmed that natural antisense S transcripts exist in leaves and seedling shoots and in all sexual tissues tested except mature pollen. These natural antisense S transcripts co-exist with several less abundant sense S transcripts. The accumulation of sense and antisense S transcripts is differentially regulated during pollen and silk development. Thus, these results support a role for S gene family members in sexual tissue development and/or compatible pollination and reveal a new level of complexity in the regulation and function of the S gene family in maize.
引用
收藏
页码:24146 / 24155
页数:10
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