Identification of genomic differences between Escherichia coli strains pathogenic for poultry and E-coli K-12 MG1655 using suppression subtractive hybridization analysis

被引:16
作者
Stocki, SL
Babiuk, LA
Rawlyk, NA
Potter, AA
Allan, BJ
机构
[1] Vet Infect Dis Org, Saskatoon, SK S7N 5E3, Canada
[2] Canadian Bacterial Dis Network, Calgary, AB, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
subtractive hybridization; avian pathogenic E. coli; ECOR reference collection;
D O I
10.1006/mpat.2002.0536
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Diseases of poultry caused by Escherichia coli result in significant economic loss every year. Specific virulence factors associated with E coli strains pathogenic for poultry have been identified, but it is likely that others remain to be identified. To identify unique DNA fragments associated with avian strains we used suppression subtractive hybridization. The genome of E coli K-12 strain MG1655 was subtracted from the genomes of two avian E coli strains resulting in the identification of 62 fragments specific to the two avian strains. Sequence homology analysis was done and four types of fragments were identified: plasmid sequences, phage sequences, sequences with known function and sequences without any currently known function. Two E coli collections, a reference collection of diverse strains (ECOR) and a collection of 41 avian isolates, were screened for the presence of 25 of the 62 fragments. We identified nine fragments present in significantly more of the avian strains than of the ECOR strains. Five fragments were in significantly more of the ECOR strains than the avian strains. These results suggested that the nine fragments could play a role in the pathogenesis of E coli as it relates to diseases of poultry. (C) 2002 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:289 / 298
页数:10
相关论文
共 34 条
  • [11] Dho-Moulin M, 1999, VET RES, V30, P299
  • [12] Suppression subtractive hybridization: A method for generating differentially regulated or tissue-specific cDNA probes and libraries
    Diatchenko, L
    Lau, YFC
    Campbell, AP
    Chenchik, A
    Moqadam, F
    Huang, B
    Lukyanov, S
    Lukyanov, K
    Gurskaya, N
    Sverdlov, ED
    Siebert, PD
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (12) : 6025 - 6030
  • [13] Relationship between the Tsh autotransporter and pathogenicity of avian Escherichia coli and localization and analysis of the tsh genetic region
    Dozois, CM
    Dho-Moulin, M
    Brée, A
    Fairbrother, JM
    Desautels, C
    Curtiss, R
    [J]. INFECTION AND IMMUNITY, 2000, 68 (07) : 4145 - 4154
  • [14] Studies on cellulitis and other disease syndromes caused by Escherichia coli in broilers in Sri Lanka
    Gomis, SM
    Gomis, AIU
    Horadagoda, NU
    Wijewardene, TG
    Allan, BJ
    Potter, AA
    [J]. TROPICAL ANIMAL HEALTH AND PRODUCTION, 2000, 32 (06) : 341 - 351
  • [15] Hayashi T, 2001, DNA RES, V8, P11, DOI 10.1093/dnares/8.1.11
  • [16] A subtractive hybridisation analysis of genomic differences between the uropathogenic E-coli strain 536 and the E-coli K-12 strain MG1655
    Janke, B
    Dobrindt, U
    Hacker, J
    Blum-Oehler, G
    [J]. FEMS MICROBIOLOGY LETTERS, 2001, 199 (01) : 61 - 66
  • [17] The EcoCyc and MetaCyc databases
    Karp, PD
    Riley, M
    Saier, M
    Paulsen, IT
    Paley, SM
    Pellegrini-Toole, A
    [J]. NUCLEIC ACIDS RESEARCH, 2000, 28 (01) : 56 - 59
  • [18] The role of fimbriae and flagella in the adherence of avian strains of Escherichia coli O78:K80 to tissue culture cells and tracheal and gut explants
    La Ragione, RM
    Cooley, WA
    Woodward, MJ
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 2000, 49 (04) : 327 - 338
  • [19] Genetic variation of dTDP-L-rhamnose pathway genes in Salmonella enterica
    Li, Q
    Reeves, PR
    [J]. MICROBIOLOGY-UK, 2000, 146 : 2291 - 2307
  • [20] Miyamoto H, 1999, DNA Res, V6, P235, DOI 10.1093/dnares/6.4.235