Diversity and host specificity of endophytic Rhizoctonia-like fungi from tropical orchids

被引:187
作者
Otero, JT [1 ]
Ackerman, JD [1 ]
Bayman, P [1 ]
机构
[1] Univ Puerto Rico, Dept Biol, Rio Piedras, PR 00931 USA
关键词
Ceratobasidium; orchid mycorrhizae; Puerto Rico; Rhizoctonia; specificity;
D O I
10.3732/ajb.89.11.1852
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
All orchids have an obligate relationship with mycorrhizal symbionts. Most orchid mycorrhizal fungi are classified in the form-genus Rhizoctonia. This group includes anamorphs of Tulasnella, Ceratobasidium, and Thanatephorus. Rhizoctonia can be classified according to the number of nuclei in young cells (multi-, bi-, and uninucleate). From nine Puerto Rican orchids we isolated 108 Rhizoctonia-like fungi. Our isolates were either bi- or uninucleate, the first report of uninucleate Rhizoctonia-like fungi as orchid endophytes. we sequenced the internal transcribed spacer (ITS) region of nuclear ribosomal DNA from 26 isolates and identified four fungal Lineages, all related to Ceratobasidium spp. from temperate regions. Most orchid species hosted more than one lineage, demonstrating considerable variation in mycorrhizal associations even among related orchid species. The uninucleate condition was not a good phylogenetic character in mycorrhizal fungi from Puerto Rico. All four lineages were represented by fungi from Tolumnia variegata. but only one lineage included fungi from lonopsis utricularioides. Tropical epiphytic orchids appear to vary in degree of specificity in their mycorrhizal interactions more than previously thought.
引用
收藏
页码:1852 / 1858
页数:7
相关论文
共 58 条
[21]  
Harley JL, 1983, MYCORRHIZAL SYMBIOSI
[22]   The mode of infection of a pathogenic uninucleate Rhizoctonia sp. in conifer seedling roots [J].
Hietala, AM .
CANADIAN JOURNAL OF FOREST RESEARCH-REVUE CANADIENNE DE RECHERCHE FORESTIERE, 1997, 27 (04) :471-480
[23]   ANAMORPHIC AND TELEOMORPHIC CHARACTERISTICS OF A UNINUCLEATE RHIZOCTONIA SP ISOLATED FROM THE ROOTS OF NURSERY GROWN CONIFER SEEDLINGS [J].
HIETALA, AM ;
SEN, R ;
LILJA, A .
MYCOLOGICAL RESEARCH, 1994, 98 :1044-1050
[24]   Molecular evidence suggests that Ceratobasidium bicorne has an anamorph known as a conifer pathogen [J].
Hietala, AM ;
Vahala, J ;
Hantula, J .
MYCOLOGICAL RESEARCH, 2001, 105 :555-562
[25]   Identification of mycorrhizal fungi from single pelotons of Dactylorhiza majalis (Orchidaceae) using single-strand conformation polymorphism and mitochondrial ribosomal large subunit DNA sequences [J].
Kristiansen, KA ;
Taylor, DL ;
Kjoller, R ;
Rasmussen, HN ;
Rosendahl, S .
MOLECULAR ECOLOGY, 2001, 10 (08) :2089-2093
[26]   Sequence variation of the rDNA ITS regions within and between anastomosis groups in Rhizoctonia solani [J].
Kuninaga, S ;
Natsuaki, T ;
Takeuchi, T ;
Yokosawa, R .
CURRENT GENETICS, 1997, 32 (03) :237-243
[27]  
Lee S.B., 1990, PCR PROTOCOLS GUIDE, DOI [DOI 10.1016/B978-0-12-372180-8.50038-X, 10.1016/B978-0-12-372180-8.50038-X]
[28]   Identification of a uninucleate Rhizoctonia sp by pathogenicity, hyphal anastomosis and RAPD analysis [J].
Lilja, A ;
Hietala, AM ;
Karjalainen, R .
PLANT PATHOLOGY, 1996, 45 (05) :997-1006
[29]   SOAP, cleaning multiple alignments from unstable blocks [J].
Löytynoja, A ;
Milinkovitch, MC .
BIOINFORMATICS, 2001, 17 (06) :573-574
[30]   IN-SITU AND IN-VITRO SPECIFICITY BETWEEN RHIZOCTONIA SPP AND SPIRANTHES-SINENSIS (PERSOON) AMES VAR AMOENA (M BIEBERSTEIN) HARA (ORCHIDACEAE) [J].
MASUHARA, G ;
KATSUYA, K .
NEW PHYTOLOGIST, 1994, 127 (04) :711-718