Characterization of a gene cluster of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lantibiotic, nukacin ISK-1

被引：31

作者：

Aso, Y ^{[1
]}

Sashihara, T ^{[1
]}

Nagao, J ^{[1
]}

Kanemasa, Y ^{[1
]}

Koga, H ^{[1
]}

Hashimoto, T ^{[1
]}

Higuchi, T ^{[1
]}

Adachi, A ^{[1
]}

Nomiyama, H ^{[1
]}

Ishizaki, A ^{[1
]}

Nakayama, J ^{[1
]}

Sonomoto, K ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch, Fac Agr,Dept Biosci & Biotechnol, Div Microbial Sci & Technol,Lab Microbial Technol, Fukuoka 8128581, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 2004年 / 68卷 / 08期

基金：

日本学术振兴会;

关键词：

lantibiotic; bacteriocin; nukacin ISK-1; Staphylococcus g; gene cluster;

D O I：

10.1271/bbb.68.1663

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We characterized a gene cluster in a plasmid designated pPI-1 of Staphylococcus warneri ISK-1 encoding the biosynthesis of and immunity to the lacticin-481 type lantibiotic, nukacin ISK-1. The DNA sequence suggested that the nukacin ISK-1 gene cluster consists of at least six genes, nukA (a structural gene), -M, -T, -F, -E, -G, and two open reading frames, ORF1 and ORF7. NukM and NukT were predicted to be involved in post-translational modification and secretion of nukacin ISK-1 respectively. NukF, -E, and -G were predicted to form a membrane complex which contributes to self-protection from nukacin ISK-1. Transcriptional analyses revealed that nukM through ORF7 comprises an operon, and that ORF1 is transcribed independently from downstream of nukA. The transcriptional levels of the nukA and nukM genes were enhanced by osmotic stress. The expression level of the nukA transcript was scarcely enhanced by nukacin ISK-1, suggesting that expression is not under the control of the autoregulatory circuit.

引用

页码：1663 / 1671

页数：9

共 33 条

[11]

Kalmokoff ML, 1999, APPL ENVIRON MICROB, V65, P2128

[12] A bacteriocin of strain Pediococcus sp. ISK-1 isolated from Nukadoko, bed of fermented rice bran [J].

Kimura, H ;

Nagano, R ;

Matsusaki, H ;

Sonomoto, K ;

Ishizaki, A .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1997, 61 (06) :1049-1051

[13] Purification and partial identification of bacteriocin ISK-1, a new lantibiotic produced by Pediococcus sp. ISK-1 [J].

Kimura, H ;

Matsusaki, H ;

Sashihara, T ;

Sonomoto, K ;

Ishizaki, A .

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1998, 62 (12) :2341-2345

[14] BIOSYNTHESIS OF THE LANTIBIOTIC SUBTILIN IS REGULATED BY A HISTIDINE KINASE RESPONSE REGULATOR SYSTEM [J].

KLEIN, C ;

KALETTA, C ;

ENTIAN, KD .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (01) :296-303

[15] AUTOREGULATION OF NISIN BIOSYNTHESIS IN LACTOCOCCUS-LACTIS BY SIGNAL-TRANSDUCTION [J].

KUIPERS, OP ;

BEERTHUYZEN, MM ;

DERUYTER, PGGA ;

LUESINK, EJ ;

DEVOS, WM .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (45) :27299-27304

[16] A SIMPLE METHOD FOR DISPLAYING THE HYDROPATHIC CHARACTER OF A PROTEIN [J].

KYTE, J ;

DOOLITTLE, RF .

JOURNAL OF MOLECULAR BIOLOGY, 1982, 157 (01) :105-132

[17] Identification and overexpression of ltnl, a novel gene which confers immunity to the two-component lantibiotic lacticin 3147 [J].

McAuliffe, O ;

Hill, C ;

Ross, RP .

MICROBIOLOGY-UK, 2000, 146 :129-138

[18] Lantibiotics: structure, biosynthesis and mode of action [J].

McAuliffe, O ;

Ross, RP ;

Hill, C .

FEMS MICROBIOLOGY REVIEWS, 2001, 25 (03) :285-308

[19] Nucleotide sequence of the streptococcin A-FF22 lantibiotic regulon:: model for production of the lantibiotic SA-FF22 by strains of Streptococcus pyogenes [J].

McLaughlin, RE ;

Ferretti, JJ ;

Hynes, WL .

FEMS MICROBIOLOGY LETTERS, 1999, 175 (02) :171-177

[20] A novel type of conserved DNA-binding domain in the transcriptional regulators of the AlgR/AgrA/LytR family [J].

Nikolskaya, AN ;

Galperin, MY .

NUCLEIC ACIDS RESEARCH, 2002, 30 (11) :2453-2459

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