Next-generation sequencing

被引：110

作者：

Reis-Filho, Jorge S. ^{[1
]}

机构：

[1] Inst Canc Res, Breakthrough Breast Canc Res Ctr, Mol Pathol Team, London SW3 6JB, England

来源：

BREAST CANCER RESEARCH | 2009年 / 11卷

关键词：

COPY-NUMBER-VARIATION; CANCER CELL-LINE; BREAST-CANCER; DUCTAL CARCINOMAS; HUMAN GENOME; TRANSCRIPTOME; RESISTANCE; MUTATION; LEUKEMIA; IDENTIFICATION;

D O I：

10.1186/bcr2431

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Next-generation sequencing (also known as massively parallel sequencing) technologies are revolutionising our ability to characterise cancers at the genomic, transcriptomic and epigenetic levels. Cataloguing all mutations, copy number aberrations and somatic rearrangements in an entire cancer genome at base pair resolution can now be performed in a matter of weeks. Furthermore, massively parallel sequencing can be used as a means for unbiased transcriptomic analysis of mRNAs, small RNAs and noncoding RNAs, genome-wide methylation assays and high-throughput chromatin immunoprecipitation assays. Here, I discuss the potential impact of this technology on breast cancer research and the challenges that come with this technological breakthrough.

引用

页数：7

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