Natural IgM antibodies in baby rabbit serum bind high-mannose glycans on HIV type 1 glycoprotein 120/160 and activate classic complement pathway

被引：10

作者：

Gerencer, M ^{[1
]}

Barrett, PN ^{[1
]}

Kistner, O ^{[1
]}

Mitterer, A ^{[1
]}

Dorner, F ^{[1
]}

机构：

[1] Immuno AG Wien, Biomed Res Ctr, A-2304 Orth, Austria

来源：

AIDS RESEARCH AND HUMAN RETROVIRUSES | 1998年 / 14卷 / 07期

关键词：

D O I：

10.1089/aid.1998.14.599

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Serum from rodents and felines has been found very effective in complement-dependent lysis of HIV-1, even in nonimmunized animals, but the effector molecules in animal serum and target structures on HIV-1 envelope gp120/160 responsible for complement activation were not determined. We have found that the natural anti-carbohydrate-specific IgM antibodies present in baby rabbit serum were able to lyse effectively the CD4(+) T cells coated with the whole virus or with a recombinant gp120/160, irrespectively of the virus strain or glycoprotein expression system. When the high mannose-type glycans on gp160 were enzymatically removed by endoglycosidase F or blocked with the specific lectins, the complement activation and subsequent cell lysis were abolished. IgM-depleted baby rabbit serum was not able to lyse the gp120/160- and/or whole virus-coated target cells. These results suggest that the target structures for complement-activating and naturally occurring IgM antibodies in baby rabbit serum are high-mannose residues on HIV-1 envelope glycoprotein.

引用

页码：599 / 605

页数：7

共 41 条

[21] COMPLEMENT ACTIVATION UPON BINDING OF MANNAN-BINDING PROTEIN TO HIV ENVELOPE GLYCOPROTEINS [J].