Simultaneous determination of mecamylamine, nicotine, and cotinine in plasma by gas chromatography-mass spectrometry

被引：51

作者：

Jacob, P

Wu, S

Yu, L

Benowitz, NL

机构：

[1] Univ Calif San Francisco, San Francisco Gen Hosp, Med Ctr,Langley Porter Psychiat Inst, Dept Med, San Francisco, CA 94110 USA

[2] Univ Calif San Francisco, San Francisco Gen Hosp, Med Ctr,Langley Porter Psychiat Inst, Drug Dependence Res Ctr,Div Clin Pharmacol, San Francisco, CA 94110 USA

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2000年 / 23卷 / 04期

关键词：

mecamylamine; nicotine; cotinine; plasma; gas chromatography; mass spectrometry;

D O I：

10.1016/S0731-7085(00)00343-5

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The nicotine receptor antagonist mecamylamine has been shown to increase the efficacy of transdermal nicotine as a pharmacotherapy for tobacco addiction. A product for simultaneous transdermal administration of nicotine and mecamylamine is undergoing clinical trials. In order to carry out pharmacokinetic studies, quantitation of low nanogram per milliliter levels of mecamylamine and nicotine was required. This paper describes a method for simultaneous determination of mecamylamine, nicotine, and the nicotine metabolite, cotinine, in human plasma using gas chromatography-mass spectrometry (GC-MS). Limits of quantitation for mecamylamine, nicotine and cotinine are 2, 1 and 2 ng/ml, respectively. (C) 2000 Elsevier Science B.V. AII rights reserved.

引用

页码：653 / 661

页数：9