Induction of apoptosis by fatty acid ethyl esters in HepG2 cells

被引：23

作者：

Aydin, HH ^{[1
]}

Celik, HA

Deveci, R

Karacali, S

Saydam, G

Omay, SB

Batur, Y

机构：

[1] Ege Univ, Sch Med, Dept Biochem, TR-35100 Izmir, Turkey

[2] Sch Sci, Dept Mol Biol & Cell Biol, TR-35100 Izmir, Turkey

[3] Ege Univ, Sch Med, Dept Hematol, TR-35100 Izmir, Turkey

[4] Ege Univ, Sch Med, Dept Gastroenterol, TR-35100 Izmir, Turkey

来源：

FOOD AND CHEMICAL TOXICOLOGY | 2005年 / 43卷 / 01期

关键词：

fatty acid ethyl esters; ethyl palmitate; ethyl stearate; apoptosis; HepG2; cells;

D O I：

10.1016/j.fct.2004.09.003

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Fatty acid ethyl esters (FAEEs) are esterification products of ethanol and fatty acids which have been found particularly in the organ damaged by ethanol abuse. To evaluate any effect of FAEEs on HepG2 cells, we added FAEEs to cell culture medium. Electrophoresis of DNA from HepG2 cells exposed to 18.5 muM ethyl palmitate (EP) and 10.6 muM ethyl stearate (ES) for 24 h revealed a smear which is typical of non-specific degradation by DNA ladder assay. Apoptosis was characterized by electron microscopy, flow cytometry revealed that the cell cycle of HepG2 cells was perturbed by exposure to FAEEs. In the present study we demonstrate that treatment of HepG2 cells with EP and ES induces apoptosis, as well as perturbing the cell cycle as the number of cells in the G(2)/M and S phases decreased. (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：139 / 145

页数：7

共 42 条

[11]

DOYLE KM, 1994, J LIPID RES, V35, P428

[12]

GRIGOR MR, 1973, BIOCHIM BIOPHYS ACTA, V306, P20

[13] Fatty acid ethyl esters, nonoxidative metabolites of ethanol, accelerate the kinetics of activation of the human brain delayed rectifier KC channel, Kv1.1 [J].

GubitosiKlug, RA ;

Gross, RW .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (51) :32519-32522

[14]

HABER PS, 1993, J LAB CLIN MED, V121, P759

[15] NONOXIDATIVE METABOLISM OF ETHANOL IN THE PANCREAS - IMPLICATION IN ALCOHOLIC PANCREATIC DAMAGE [J].

HAMAMOTO, T ;

YAMADA, S ;

HIRAYAMA, C .

BIOCHEMICAL PHARMACOLOGY, 1990, 39 (02) :241-245

[16] Protective effects of carnosine against protein modification mediated by malondialdehyde and hypochlorite [J].

Hipkiss, AR ;

Worthington, VC ;

Himsworth, DTJ ;

Herwig, W .

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1998, 1380 (01) :46-54

[17] Acetaldehyde cytotoxicity in cultured rat astrocytes [J].

Holownia, A ;

Ledig, M ;

Braszko, JJ ;

Ménez, JF .

BRAIN RESEARCH, 1999, 833 (02) :202-208

[18] Effects of fatty acids on liver metastasis of ACL-15 rat colon cancer cells [J].

Iwamoto, S ;

Senzaki, H ;

Kiyozuka, Y ;

Ogura, E ;

Takada, H ;

Hioki, K ;

Tsubura, A .

NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 1998, 31 (02) :143-150

[19] EFFECTS OF FATTY-ACIDS ON HEPATIC GENE-EXPRESSION [J].

JUMP, DB ;

REN, B ;

CLARKE, S ;

THELEN, A .

PROSTAGLANDINS LEUKOTRIENES AND ESSENTIAL FATTY ACIDS, 1995, 52 (2-3) :107-111

[20] Purification and characterization of rat liver microsomal fatty acid ethyl and 2-chloroethyl ester synthase and their relationship with carboxylesterase (pI 6.1) [J].

Kaphalia, BS ;

Fritz, RR ;

Ansari, GAS .

CHEMICAL RESEARCH IN TOXICOLOGY, 1997, 10 (02) :211-218

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