Neuroprotective Effect of Honokiol and Magnolol, Compounds from Magnolia officinalis, on Beta-amyloid-induced Toxicity in PC12 Cells

Amyloid beta peptide (A beta) induced toxicity is a well-established pathway of neuronal cell death which might play a role in Alzheimer's disease. In this regard, the toxic effect of A beta on a cultured A beta-sensitive neuronal cell line was used as a primary screening tool for potential anti-Alzheimer's therapeutic agents. The effects of nine pure compounds (vitamin E, alpha-asarone, salidroside, baicolin, magnolol, gastrodin, bilobalide, honokiol and beta-asarone) from selected Chinese herbs on neuronal cell death induced by A beta in NGF-differentiated PC12 cells were examined. Only two of the studied compounds, honokiol and magnolol, significantly decreased A beta-induced cell death. Further experiments indicated that their neuroprotective effects are possibly mediated through reduced ROS production as well as suppression of intracellular calcium elevation and inhibition of caspase-3 activity. The results provide for the first time a scientific rationale for the clinical use of honokiol and magnolol in the treatment of Alzheimer's disease. Copyright (c) 2010 John Wiley & Sons, Ltd.