Thyroid-Stimulating Hormone, a Novel, Locally Produced Modulator of Human Epidermal Functions, Is Regulated by Thyrotropin-Releasing Hormone and Thyroid Hormones

被引:76
作者
Bodo, Eniko [1 ,3 ]
Kany, Benedikt [1 ]
Gaspar, Erzsebet [1 ]
Knuever, Jana [1 ]
Kromminga, Arno [4 ]
Ramot, Yuval [1 ,5 ]
Biro, Tamas [6 ,7 ]
Tiede, Stephan [1 ]
van Beek, Nina [1 ]
Poeggeler, Burkhard [1 ]
Meyer, Katja C. [1 ]
Wenzel, Bjorn E. [2 ]
Paus, Ralf [1 ,8 ]
机构
[1] Univ Lubeck, Dept Dermatol, D-23538 Lubeck, Germany
[2] Univ Lubeck, Dept Med 1, D-23538 Lubeck, Germany
[3] Coll Nyiregyhaza, Agr & Mol Res Inst, H-4400 Nyiregyhaza, Hungary
[4] IPM Biotech, D-22339 Hamburg, Germany
[5] Hadassah Hebrew Univ, Dept Dermatol, Med Ctr, IL-91240 Jerusalem, Israel
[6] Univ Debrecen, Dept Physiol, H-4032 Debrecen, Hungary
[7] Abiolt Ltd, H-4032 Debrecen, Hungary
[8] Univ Manchester, Sch Translat Med, Manchester M13 9PT, Lancs, England
关键词
HUMAN HAIR FOLLICLE; HUMAN-SKIN; IN-VITRO; MONOCLONAL-ANTIBODIES; EXPRESSION; GROWTH; PROLIFERATION; FEEDBACK; RECEPTOR; AXIS;
D O I
10.1210/en.2009-0306
中图分类号
R5 [内科学];
学科分类号
100201 [内科学];
摘要
Several elements of the hypothalamic-pituitary-thyroid axis (HPT) reportedly are transcribed by human skin cell populations, and human hair follicles express functional receptors for TSH. Therefore, we asked whether the epidermis of normal human skin is yet another extrathyroidal target of TSH and whether epidermis even produces TSH. If so, we wanted to clarify whether intraepidermal TSH expression is regulated by TRH and/or thyroid hormones and whether TSH alters selected functions of normal human epidermis in situ. TSH and TSH receptor (TSH-R) expression were analyzed in the epidermis of normal human scalp skin by immunohistochemistry and PCR. In addition, full-thickness scalp skin was organ cultured and treated with TSH, TRH, or thyroid hormones, and the effect of TSH treatment on the expression of selected genes was measured by quantitative PCR and/or quantitative immunohistochemistry. Here we show that normal human epidermis expresses TSH at the mRNA and protein levels in situ and transcribes TSH-R. It also contains thyrostimulin transcripts. Intraepidermal TSH immunoreactivity is up-regulated by TRH and down-regulated by thyroid hormones. Although TSH-R immunoreactivity in situ could not be documented within the epidermis, but in the immediately adjacent dermis, TSH treatment of organ-cultured human skin strongly up-regulated epidermal expression of involucrin, loricrin, and keratins 5 and 14. Thus, normal human epidermis in situ is both an extrapituitary source and (possibly an indirect) target of TSH signaling, which regulates defined epidermal parameters. Intraepidermal TSH expression appears to be regulated by the classical endocrine controls that determine the systemic HPT axis. (Endocrinology 151: 1633-1642, 2010)
引用
收藏
页码:1633 / 1642
页数:10
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