Chitosan-based hydrogels do not induce angiogenesis

被引：30

作者：

Ahmadi, Raheleh ^{[1
]}

Burns, Alan J. ^{[2
]}

de Bruijn, Joost D. ^{[1
]}

机构：

[1] Queen Mary Univ London, Sch Mat Sci & Engn, London E1 4NS, England

[2] UCL Inst Child Hlth, London WC1N 1EH, England

来源：

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE | 2010年 / 4卷 / 04期

关键词：

chitosan-glycerol phosphate-hydroxyethyl cellulose; biphasic calcium phosphate microparticles; chick chorioallantoic membrane assay; angiogenesis; mesenchymal stem cells; injectable bone tissue engineering; ENDOTHELIAL GROWTH-FACTOR; MARROW STROMAL CELLS; IN-VIVO; BONE-FORMATION; SCAFFOLDS; PROLIFERATION; VITRO; VASCULARIZATION; EXPRESSION; CARTILAGE;

D O I：

10.1002/term.247

中图分类号：

Q813 [细胞工程];

学科分类号：

100113 [医学细胞生物学];

摘要：

The aim of this study was to assess the angiogenic potential of chitosan-glycerol phosphate (GP)-hydroxyethyl cellulose (HEC) binder for injectable bone tissue engineering applications. The angiogenic response of chitosan-GP-HEC combined with and without human bone marrow-derived mesenchymal stem cells (hMSCs) was examined using the chick chorioallantoic membrane (CAM) assay. Chitosan-GP-HEC gel did not show any angiogenic potential, whereas the presence of hMSCs gave rise to an enhanced angiogenic response when placed on the CAM for 3 days. Quantitatively, significantly more blood vessel formation was observed for the stem cell-containing group as compared to all other groups (p < 0.05), except for the b-FGF-positive control. The results indicate that the chitosan-GP-HEC binder does not contribute to enhanced angiogenesis and that the presence of hMSCs enhances angiogenesis. Copyright (C) 2009 John Wiley & Sons, Ltd.

引用

页码：309 / 315

页数：7

共 54 条

[1]

Capillary vessel network integration by inserting a vascular pedicle enhances bone formation in tissue-engineered bone using interconnected porous hydroxyapatite ceramics [J].