Plant transformation technology -: Developments and applications

被引：87

作者：

Newell, CA ^{[1
]}

机构：

[1] Univ Cambridge, Dept Plant Sci, Cambridge CB2 3EA, England

来源：

MOLECULAR BIOTECHNOLOGY | 2000年 / 16卷 / 01期

关键词：

plant transformation; Agrobacterium; direct DNA delivery; particle bombardment; plastid transformation;

D O I：

10.1385/MB:16:1:53

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant transformation has its roots in the research on Agrobacterium that was being undertaken in the early 1980s. The last two decades have seen significant developments in plant transformation technology, such that a large number of transgenic crop plants have now been released for commercial production. Advances in the technology have been due to development of a range of Agrobacterium-mediated and direct DNA delivery techniques, along with appropriate tissue culture techniques for regenerating whole plants from plant cells or tissues in a large number of species. In addition, parallel developments in molecular biology have greatly extended the range of investigations to which plant transformation technology can be applied. Research in plant transformation is concentrating now not so much on the introduction of DNA into plant cells, but rather more on the problems associated with stable integration and reliable expression of the DNA once it has been integrated.

引用

页码：53 / 65

页数：13

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