Identification of Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) by polymerase chain reaction-restriction fragment length polymorphism of a 12S rRNA gene fragment

被引：20

作者：

Asensio, L ^{[1
]}

González, I ^{[1
]}

Fernández, A ^{[1
]}

Céspedes, A ^{[1
]}

Hernández, PE ^{[1
]}

García, T ^{[1
]}

Martín, R ^{[1
]}

机构：

[1] Univ Complutense, Fac Vet, Dept Nutr & Bromatol Hyg & Tecnol Los Alimentos 3, E-28040 Madrid, Spain

来源：

JOURNAL OF FOOD PROTECTION | 2000年 / 63卷 / 09期

关键词：

D O I：

10.4315/0362-028X-63.9.1248

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Restriction site analysis of polymerase chain reaction (PCR) products from a conserved region of the 12S rRNA gene has been used for the specific identification of Nile perch (Lares niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus). Amplification of DNA isolated from muscle samples was carried out using a set of primers flanking a region of 436 bp from the mitochondrial 12S rRNA gene. Digestions of the PCR products with RsaI and Sau96I endonucleases, followed by agarose gel electrophoresis of the digested PCR products, yielded specific profiles that enabled direct identification of each species analyzed.

引用

页码：1248 / 1252

页数：5

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