Integrase-defective lentiviral vectors: progress and applications

被引：100

作者：

Banasik, M. B. ^{[1
,2
]}

McCray, P. B., Jr. ^{[1
,2
]}

机构：

[1] Univ Iowa, Dept Pediat, Carver Coll Med, Program Gene Therapy, Iowa City, IA 52242 USA

[2] Univ Iowa, Interdisciplinary Grad Program Genet, Carver Coll Med, Iowa City, IA 52242 USA

来源：

GENE THERAPY | 2010年 / 17卷 / 02期

关键词：

HIV; FIV; episome; zinc-finger nuclease; vaccine; IMMUNODEFICIENCY-VIRUS TYPE-1; TRANSIENT GENE-EXPRESSION; UNINTEGRATED VIRAL-DNA; ZINC-FINGER NUCLEASES; RETROVIRAL VECTORS; HIV-1; INTEGRASE; TRANSGENE EXPRESSION; EPISOMAL VECTORS; SITE SELECTION; HUMAN GENOME;

D O I：

10.1038/gt.2009.135

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Lentiviral vectors (LVs) offer the advantages of a large packaging capacity, broad cell tropism or specific cell-type targeting through pseudotyping, and long-term expression from integrated gene cassettes. However, transgene integration carries a risk of disrupting gene expression through insertional mutagenesis and may not be required for all applications. A non-integrating LV may be beneficial in cases in which transient gene expression is desired. Several recent publications outline the development and initial biological characterization of such vectors. Here, we discuss the potential applications and new directions for the development of integration-defective LVs. Gene Therapy (2010) 17, 150-157; doi: 10.1038/gt.2009.135; published online 22 October 2009

引用

页码：150 / 157

页数：8

共 87 条

[1] Stable gene transfer to muscle using non-integrating lentiviral vectors [J].