Gel electrophoretic detection of 7,8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine via oxidation by Ir(IV)

被引：100

作者：

Muller, JG ^{[1
]}

Duarte, V ^{[1
]}

Hickerson, RP ^{[1
]}

Burrows, CJ ^{[1
]}

机构：

[1] Univ Utah, Dept Chem, Salt Lake City, UT 84112 USA

来源：

NUCLEIC ACIDS RESEARCH | 1998年 / 26卷 / 09期

关键词：

D O I：

10.1093/nar/26.9.2247

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two gel electrophoretic methods are described for detection of 7,8-dihydro-8-oxoguanine and 7,8-dihydro-8-oxoadenine based on their further oxidation with one-electron oxidants including IrCl62- and IrBr62-. The products of nucleobase oxidation lead to enhanced piperidine-sensitive cleavage and to highly visible stop points in a primer extension assay. 8-oxoG and 8-oxoA lesions may be distinguished by the latter's inability to be oxidized by IrBr62- compared to IrCl62-. Comparison is also made to oxidation by MnO4-.

引用

页码：2247 / 2249

页数：3

共 23 条

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