Ethanol production through simultaneous saccharification and fermentation of switchgrass using Saccharomyces cerevisiae D5A and thermotolerant Kluyveromyces marxianus IMB strains

被引：77

作者：

Faga, Brian A. ^{[1
]}

Wilkins, Mark R. ^{[1
]}

Banat, Ibrahim M. ^{[2
]}

机构：

[1] Oklahoma State Univ, Dept Biosyst Engn, Stillwater, OK 74078 USA

[2] Univ Ulster, Sch Biomed Sci, Coleraine BT52 1SA, Londonderry, North Ireland

来源：

BIORESOURCE TECHNOLOGY | 2010年 / 101卷 / 07期

关键词：

Thermotolerant; Biofuels; Cellulase; KLUVEROMYCES-MARXIANUS; BIOMASS; TEMPERATURE; CELLULASE; GROWTH; YEAST;

D O I：

10.1016/j.biortech.2009.11.001

中图分类号：

S2 [农业工程];

学科分类号：

0828 ;

摘要：

Hydrothermolysis pretreated switchgrass at 200 degrees C for 10 min was used in a Simultaneous saccharification and fermentation (SSF) process using five thermotolerant yeast strains Kluyveromyces marxianus IMB 1, IMB 2, IMB 3, IMB 4, and IMB 5 at 45 degrees C and Saccharomyces cerevisiae D(5)A at 37 degrees C. SSF was carried out for 7 d using 5, 10, and 15 FPU/g glucan to determine the effect of decreasing cellulase loading on ethanol yield. The effect of initial pH on SSF by S. cerevisiae D(5)A was also investigated. Fermentation by K. marxianus IMB 1. IMB 2, IMB 4, and IMB 5 ceased by 72 h and fermentation by K. marxianus IMB 3 ceased by 96 h, while fermentation S. cerevisiae D(5)A continued for 7 d. At 96 and 120 h, IMB 3 and S. cerevisiae D(5)A had similar ethanol yields while the other K. marxianus strains were lower at a 95% confidence level. Final ethanol yields for IMB 3, IMB 1, IMB 5 strains were similar to one another, however, ethanol yield for S. cerevisiae D(5)A (92% maximum theoretical) was greater than all of the IMB strains except IMB 3 at a 95% confidence level. Reducing enzyme loading reduced ethanol yields for both K. marxianus IMB 3 and S. cerevisiae D(5)A. Reducing buffer pH from 5.5 to 4.8 reduced ethanol yields for S. cerevisiae D(5)A. This study shows that K. marxianus IMB 3 has potential for commercial use for ethanol production from cellulose in SSF processes with further improvement of its thermotolerance. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：2273 / 2279

页数：7

共 23 条

[1]

[Anonymous], 2005, NATL RENEW ENERGY LA

[2] Ethanol from lignocellulosic materials by a simultaneous saccharification and fermentation process (SFS) with Kluyveromyces marxianus CECT 10875 [J].

Ballesteros, M ;

Oliva, JM ;

Negro, MJ ;

Manzanares, P ;

Ballesteros, I .

PROCESS BIOCHEMISTRY, 2004, 39 (12) :1843-1848

[3] ISOLATION OF THERMOTOLERANT, FERMENTATIVE YEASTS GROWING AT 52-DEGREES-C AND PRODUCING ETHANOL AT 45-DEGREES-C AND 50-DEGREES-C [J].

BANAT, IM ;

NIGAM, P ;

MARCHANT, R .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 1992, 8 (03) :259-263

[4]

BARRON N, 1995, APPL MICROBIOL BIOT, V43, P518, DOI [10.1007/BF00218459, 10.1007/s002530050444]

[5]

Barron N, 1997, BIOPROCESS ENG, V17, P383, DOI 10.1007/s004490050401

[6] Simultaneous saccharification and fermentation of steam-pretreated spruce to ethanol [J].

Bollók, M ;

Réczey, K ;

Zacchi, G .

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 2000, 84-6 (1-9) :69-80

[7]

Dowe N., 2001, SSF Experimental Protocols - Lignocellulosic Biomass Hydrolysis and Fermentation

[8] FUNCTION OF LIPOPHILIC ACIDS AS ANTIMICROBIAL FOOD ADDITIVES [J].

FREESE, E ;

SHEU, CW ;

GALLIERS, E .

NATURE, 1973, 241 (5388) :321-325

[9] MEASUREMENT OF CELLULASE ACTIVITIES [J].

GHOSE, TK .

PURE AND APPLIED CHEMISTRY, 1987, 59 (02) :257-268

[10] THE EFFECT OF TEMPERATURE ON THE KINETICS OF ETHANOL-PRODUCTION BY A THERMOTOLERANT STRAIN OF KLUVEROMYCES-MARXIANUS [J].

HUGHES, DB ;

TUDROSZEN, NJ ;

MOYE, CJ .

BIOTECHNOLOGY LETTERS, 1984, 6 (01) :1-6

← 1 2 3 →