Double stranded DNA sequencing by tandem mass spectrometry

被引：26

作者：

McLafferty, FW ^{[1
]}

Aaserud, DJ ^{[1
]}

Guan, ZQ ^{[1
]}

Little, DP ^{[1
]}

Kelleher, NL ^{[1
]}

机构：

[1] Cornell Univ, Baker Lab Chem, Ithaca, NY 14853 USA

来源：

INTERNATIONAL JOURNAL OF MASS SPECTROMETRY | 1997年 / 165卷

关键词：

electrospray; Fourier-transform MS; DNA; tandem; sequencing;

D O I：

10.1016/S0168-1176(97)00171-7

中图分类号：

O64 [物理化学（理论化学）、化学物理学]; O56 [分子物理学、原子物理学];

学科分类号：

070203 ; 070304 ; 081704 ; 1406 ;

摘要：

Electrospray ionization produces far more abundant molecular ions for double stranded (ds) DNA than for single stranded (ss) and accurate molecular masses can provide the base composition of dsDNA. This study shows that the Fourier-transform mass spectra of a ds 64-mer DNA can provide approximately 50% more sequence information than the spectra of either individual ss component. Cleavages triggered by T loss are of low probability, but the opposite is true in the complementary ss strand that bears the base A at this site. The spectrum of the product of the attempted biological synthesis of a ds 70-mer DNA provided critical information on its extensive 3'-end heterogeneity. Published by Elsevier Science B.V.

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收藏

页码：457 / 466

页数：10

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