Targeted Photoswitchable Probe for Nanoscopy of Biological Structures

被引：14

作者：

Dellagiacoma, Claudio ^{[2
]}

Lukinavicius, Grazvydas ^{[1
]}

Bocchio, Noelia ^{[2
]}

Banala, Sambashiva ^{[1
]}

Geissbuehler, Stefan ^{[2
]}

Maerki, Iwan ^{[2
]}

Johnsson, Kai ^{[1
]}

Lasser, Theo ^{[2
]}

机构：

[1] Ecole Polytech Fed Lausanne, Ingn Prot Lab, CH-1015 Lausanne, Switzerland

[2] Ecole Polytech Fed Lausanne, Lab Opt Biomed, CH-1015 Lausanne, Switzerland

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 10期

基金：

瑞士国家科学基金会;

关键词：

fluorescent probes; microtubules; protein structures; SNAP-tag; STORM; OPTICAL RECONSTRUCTION MICROSCOPY; FUSION PROTEINS; FLUORESCENT-PROBES; LIVING CELLS; STORM; FLUOROPHORES; RESOLUTION; LIMIT; TAG;

D O I：

10.1002/cbic.201000189

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Figure Presented) SNAP-tag: We introduce a photoswitchable O6-benzylguanine derivative and demonstrate its use for super-resolution microscopy of SNAP-tagged proteins based on single fluorophore localization. Stochastic Optical Reconstruction Microscopy (STORM) reveals SNAP-tagged microtubule structures with 25 nm resolution. The described probe in combination with the versatile SNAP-tag labeling opens new possibilities for imaging biological structures at the nanoscale. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：1361 / 1363

页数：3

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