Shifting the optimal stiffness for cell migration

被引:243
作者
Bangasser, Benjamin L. [1 ]
Shamsan, Ghaidan A. [1 ]
Chan, Clarence E. [1 ]
Opoku, Kwaku N. [1 ]
Tuzel, Erkan [1 ,3 ]
Schlichtmann, Benjamin W. [1 ]
Kasim, Jesse A. [1 ]
Fuller, Benjamin J. [1 ]
McCullough, Brannon R. [1 ,4 ]
Rosenfeld, Steven S. [2 ]
Odde, David J. [1 ]
机构
[1] Univ Minnesota, Dept Biomed Engn, 312 Church St SE, Minneapolis, MN 55455 USA
[2] Cleveland Clin, Brain Tumor & Neurooncol Ctr, 9500 Euclid Ave, Cleveland, OH 44195 USA
[3] Worcester Polytech Inst, Dept Phys, 100 Inst Rd, Worcester, MA 01609 USA
[4] No Arizona Univ, Dept Chem & Biochem, 700 S Osborne Dr, Flagstaff, AZ 86011 USA
基金
美国国家科学基金会; 美国国家卫生研究院;
关键词
MOTOR-CLUTCH MODEL; FORCE TRANSMISSION; TRACTION; ADHESION; RIGIDITY; MATRIX; ACTIN; SUBSTRATE; MOVEMENT; DYNAMICS;
D O I
10.1038/ncomms15313
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];
摘要
Cell migration, which is central to many biological processes including wound healing and cancer progression, is sensitive to environmental stiffness, and many cell types exhibit a stiffness optimum, at which migration is maximal. Here we present a cell migration simulator that predicts a stiffness optimum that can be shifted by altering the number of active molecular motors and clutches. This prediction is verified experimentally by comparing cell traction and F-actin retrograde flow for two cell types with differing amounts of active motors and clutches: embryonic chick forebrain neurons (ECFNs; optimum similar to 1 kPa) and U251 glioma cells (optimum similar to 100 kPa). In addition, the model predicts, and experiments confirm, that the stiffness optimum of U251 glioma cell migration, morphology and F-actin retrograde flow rate can be shifted to lower stiffness by simultaneous drug inhibition of myosin II motors and integrin-mediated adhesions.
引用
收藏
页数:10
相关论文
共 31 条
[1]
Alt W, 1990, BIOLOGICAL MOTION, V89, P254
[2]
[Anonymous], 2009, Theory of Elasticity
[3]
PROTEIN-DERIVATIZED GLASS COVERSLIPS FOR THE STUDY OF CELL-TO-SUBSTRATUM ADHESION [J].
APLIN, JD ;
HUGHES, RC .
ANALYTICAL BIOCHEMISTRY, 1981, 113 (01) :144-148
[4]
Master Equation-Based Analysis of a Motor-Clutch Model for Cell Traction Force [J].
Bangasser, Benjamin L. ;
Odde, David J. .
CELLULAR AND MOLECULAR BIOENGINEERING, 2013, 6 (04) :449-459
[5]
Determinants of Maximal Force Transmission in a Motor-Clutch Model of Cell Traction in a Compliant Microenvironment [J].
Bangasser, Benjamin L. ;
Rosenfeld, Steven S. ;
Odde, David J. .
BIOPHYSICAL JOURNAL, 2013, 105 (03) :581-592
[6]
The role of myosin II in glioma invasion of the brain [J].
Beadle, Christopher ;
Assanah, Marcela C. ;
Monzo, Pascale ;
Vallee, Richard ;
Rosenfeld, Steven S. ;
Canoll, Peter .
MOLECULAR BIOLOGY OF THE CELL, 2008, 19 (08) :3357-3368
[7]
BELL GI, 1978, SCIENCE, V200, P618, DOI 10.1126/science.347575
[8]
Traction fields, moments, and strain energy that cells exert on their surroundings [J].
Butler, JP ;
Tolic-Norrelykke, IM ;
Fabry, B ;
Fredberg, JJ .
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, 2002, 282 (03) :C595-C605
[9]
Traction Dynamics of Filopodia on Compliant Substrates [J].
Chan, Clarence E. ;
Odde, David J. .
SCIENCE, 2008, 322 (5908) :1687-1691
[10]
Substrate stress relaxation regulates cell spreading [J].
Chaudhuri, Ovijit ;
Gu, Luo ;
Darnell, Max ;
Klumpers, Darinka ;
Bencherif, Sidi A. ;
Weaver, James C. ;
Huebsch, Nathaniel ;
Mooney, David J. .
NATURE COMMUNICATIONS, 2015, 6