The Botrytis cinerea aspartic proteinase family

被引：74

作者：

ten Have, Arjen ^{[1
,2
]}

Espino, Jose J. ^{[3
]}

Dekkers, Ester ^{[2
]}

Van Sluyter, Steven C. ^{[4
,5
]}

Brito, Nelida ^{[3
]}

Kay, John ^{[6
]}

Gonzalez, Celedonio ^{[3
]}

van Kan, Jan A. L. ^{[2
]}

机构：

[1] Univ Nacl Mar del Plata, Inst Invest Biol, CONICET, Fac Ciencias Exactas & Nat, RA-7600 Mar Del Plata, Argentina

[2] Wageningen Univ, Phytopathol Lab, NL-6700 EE Wageningen, Netherlands

[3] Univ La Laguna, Dept Bioquim & Biol Mol, E-38206 Tenerife, Spain

[4] Australian Wine Res Inst, Urrbrae, SA 5064, Australia

[5] Univ Melbourne, Sch Bot, Melbourne, Vic 3010, Australia

[6] Cardiff Univ, Sch Biosci, Cardiff CF10 3AX, S Glam, Wales

来源：

FUNGAL GENETICS AND BIOLOGY | 2010年 / 47卷 / 01期

关键词：

Gray mould; Bioinformatics; Proteinase; Plant pathogen; Phylogeny; Evolution; CELL-WALL; SACCHAROMYCES-CEREVISIAE; TRICHODERMA-HARZIANUM; PROTEOMIC ANALYSIS; PORCINE PEPSIN; GENE; PEPTIDASES; INFECTION; VIRULENCE; CLONING;

D O I：

10.1016/j.fgb.2009.10.008

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The ascomycete plant pathogen Botrytis cinerea secretes aspartic proteinase (AP) activity. Functional analysis was carried out on five aspartic proteinase genes (Bcapl-5) reported previously. Single and double mutants lacking these five genes showed neither a reduced secreted proteolytic activity, nor a reduction in virulence and they showed no alteration in sensitivity to antifungal proteins purified from grape juice. Scrutiny of the B. cinerea genome revealed the presence of nine additional Bcap genes, denoted Bcap6-14. The product of the Bcap8 gene was found to constitute up to 23% of the total protein secreted by B. cinerea. Bcap8-deficient mutants secreted similar to 70% less AP activity but were just as virulent as the wild-type strain. Phylogenetic analysis showed that Bcap8 has orthologs in many basidiomycetes but only few ascomycetes including the biocontrol fungus Trichoderma harzanium. Potential functions of the 14 APs in B. cinerea are discussed based on their sequence characteristics, phylogeny and predicted localization. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：53 / 65

页数：13

共 61 条

[1] REVISED 2.3-A STRUCTURE OF PORCINE PEPSIN - EVIDENCE FOR A FLEXIBLE SUBDOMAIN [J].