Flexible polyimide microelectrode array for in vivo recordings and current source density analysis

被引:173
作者
Cheung, Karen C. [1 ]
Renaud, Philippe
Tanila, Heikki
Djupsund, Kaj
机构
[1] Univ British Columbia, Dept Elect & Comp Engn, Vancouver, BC V5Z 1M9, Canada
[2] Ecole Polytech Fed Lausanne, Lab Microsyst, Lausanne, Switzerland
[3] Univ Kuopio, Dept Neurol & Neurosci, FIN-70211 Kuopio, Finland
基金
芬兰科学院;
关键词
flexible microelectrode array; in vivo recording; laminar analysis; current source density;
D O I
10.1016/j.bios.2006.08.035
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
This work presents implantable, flexible polymer-based probes with embedded microelectrodes for acute and chronic neural recordings in vivo, as tested on rodents. Acute recordings using this array were done in mice under urethane anesthesia and compared to those made using silicon-based probes manufactured at the Center for Neural Communication Technology, University of Michigan. The two electrode arrays yielded similar results. Recordings with chronically implanted polymer-based electrodes were performed for 60 days post-surgically in awake, behaving rats. The microelectrodes were used to monitor local field potentials and capture laminar differences in function of cortex and hippocampus, and produced response waveforms of undiminished amplitude and signal-to-noise ratios 8 weeks after chronic implantation. The polymer-based electrodes could also be connected to a lesion current to mark specific locations in the tissue. Current source density (CSD) analysis from the recordings depicted a source-sink-composition. Tissue response was assessed 8 weeks after insertion by immunochemical labeling with glial fibrillary acidic protein (GFAP) to identify astrocytes, and histological analysis showed minimal tissue reaction to the implanted structures. (c) 2006 Elsevier B.V. All rights reserved.
引用
收藏
页码:1783 / 1790
页数:8
相关论文
共 59 条
[21]   Apical and basal orthodromic population spikes in hippocampal CA1 in vivo show different origins and patterns of propagation [J].
Kloosterman, F ;
Peloquin, P ;
Leung, LS .
JOURNAL OF NEUROPHYSIOLOGY, 2001, 86 (05) :2435-2444
[22]   Two reentrant pathways in the hippocampal-entorhinal system [J].
Kloosterman, F ;
van Haeften, T ;
da Silva, FHL .
HIPPOCAMPUS, 2004, 14 (08) :1026-1039
[23]  
LEMON R, 1973, BRAIN UNIT ACTIVITY
[24]   LONG-TERM POTENTIATION INDUCED BY PATTERNED STIMULATION OF THE COMMISSURAL PATHWAY TO HIPPOCAMPAL CA1 REGION IN FREELY MOVING RATS [J].
LEUNG, LS ;
SHEN, B ;
KAIBARA, T .
NEUROSCIENCE, 1992, 48 (01) :63-74
[25]   ENTORHINAL INPUTS TO HIPPOCAMPAL CA1 AND DENTATE GYRUS IN THE RAT - A CURRENT-SOURCE-DENSITY STUDY [J].
LEUNG, LS ;
ROTH, L ;
CANNING, KJ .
JOURNAL OF NEUROPHYSIOLOGY, 1995, 73 (06) :2392-2403
[26]   POTENTIALS-EVOKED BY ALVEAR TRACT IN HIPPOCAMPAL CA1 REGION OF RATS .2. SPATIAL FIELD ANALYSIS [J].
LEUNG, LS .
JOURNAL OF NEUROPHYSIOLOGY, 1979, 42 (06) :1571-1589
[27]  
LEUNG LS, 1990, NEUROPHYSIOLOGICAL T
[28]  
Liu P, 1997, HIPPOCAMPUS, V7, P296, DOI 10.1002/(SICI)1098-1063(1997)7:3<296::AID-HIPO5>3.0.CO
[29]  
2-J
[30]  
Liu P, 1997, HIPPOCAMPUS, V7, P389, DOI 10.1002/(SICI)1098-1063(1997)7:4<389::AID-HIPO4>3.3.CO